Recombinant Human GABARAP protein (ab151871)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Suitable for: HPLC, SDS-PAGE

Description

  • Product name

    Recombinant Human GABARAP protein
    See all GABARAP proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    Purity is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. ab151871 was lyophilised from a 0.2 µM filtered solution.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    Escherichia coli

  • Accession

    O95166

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MKFVYKEEHPFEKRRSEGEKIRKKYPDRVPVIVEKAPKARIGDLDKKKYL VPSDLTVGQFYFLIRKRIHLRAEDALFFFVNNVIPPTSATMGQLYQEHHE EDFFLYIAYSDESVYGL

    • Predicted molecular weight

      14 kDa

    • Amino acids

      1 to 117

Specifications

Our Abpromise guarantee covers the use of ab151871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    HPLC

    SDS-PAGE

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C.

    pH: 7.50
    Constituents: 0.79% Tris HCl, 1.17% Sodium chloride

  • Reconstitution
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting. Dissolve the lyophilised protein in 1X PBS. It is not recommended to reconstitute to a concentration less than 100 µg/ml.
    Reconstituted protein solution can be stored at 4-7°C for 2-7 days. For long term storage aliquot and store at < -20°C.

General Info

  • Alternative names

    • ATG8A
    • FLC 3B
    • FLC3B
    • FLJ25768
    • GABA type A receptor associated protein
    • GABA(A) receptor associated protein
    • GABA(A) receptor-associated protein
    • GABARAP
    • GABARAP a
    • Gamma aminobutyric acid receptor associated protein
    • Gamma-aminobutyric acid receptor-associated protein
    • GBRAP_HUMAN
    • MGC120154
    • MGC120155
    • MM 46
    • MM46
    see all

  • Function

    Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.

  • Tissue specificity

    Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.

  • Sequence similarities

    Belongs to the ATG8 family.

  • Post-translational
    modifications

    The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II.

  • Cellular localization

    Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity).
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab151871? Please let us know so that we can cite the reference in this datasheet.

ab151871 has not yet been referenced specifically in any publications.

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