Recombinant Human GAD65 protein (ab114208)

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Description

  • Product name

    Recombinant Human GAD65 protein
    See all GAD65 proteins and peptides

  • Expression system

    Wheat germ

  • Accession

    Q05329

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MASPGSGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYG DAEKPAESGGSQPPRAAARKAACAC DQKPCSCSKVDVNYAFLHATDLL PACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWE LA DQPQNLEEILMHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWL TSTANTNMFTYEIAPVFVLLEYVTLKKMR EIIGWPGGSGDGIFSPGGA ISNMYAMMIARFKMFPEVKEKGMAALPRLIAFTSEHSHFSLKKGAAALGI GTDSVI LIKCDERGKMIPSDLERRILEAKQKGFVPFLVSATAGTTVYG AFDPLLAVADICKKYKIWMHVDAAWGGGLLMSR KHKWKLSGVERANSV TWNPHKMMGVPLQCSALLVREEGLMQNCNQMHASYLFQQDKHYDLSYDTG DKALQCGRHV DVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNR EGYEMVFDGKPQHTNVCFWYIPPSLRTLEDNEERMSR LSKVAPVIKAR MMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL

    • Predicted molecular weight

      90 kDa including tags

    • Amino acids

      1 to 585

Specifications

Our Abpromise guarantee covers the use of ab114208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    ELISA

    Western blot

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • 65 kDa glutamic acid decarboxylase
    • DCE 2
    • DCE2
    • DCE2_HUMAN
    • GAD 2
    • GAD 65
    • GAD-2
    • GAD-65
    • Gad2
    • Glutamate decarboxylase 2
    • Glutamate decarboxylase 2 (pancreas)
    • Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa)
    • Glutamate Decarboxylase 65
    • Glutamate decarboxylase 65 kDa isoform
    • Glutamic Acid Decarboxylase 2
    • Glutamic Acid Decarboxylase 65
    • MGC161605
    • MGC161607
    see all

  • Function

    Catalyzes the production of GABA.

  • Sequence similarities

    Belongs to the group II decarboxylase family.

  • Post-translational
    modifications

    Phosphorylated; which does not affect kinetic parameters or subcellular location.
    Palmitoylated; which is required for presynaptic clustering.

  • Cellular localization

    Cytoplasm > cytosol. Cytoplasmic vesicle. Cell junction > synapse > presynaptic cell membrane. Golgi apparatus membrane. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human GAD65 protein (ab114208)
    SDS-PAGE - Recombinant Human GAD65 protein (ab114208)
    12.5% SDS-PAGE analysis of ab114208 stained with Coomassie Blue.

References

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