Recombinant Human GAD65 protein (ab114208)

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SDS-PAGE - Recombinant Human GAD65 protein (ab114208)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: SDS-PAGE, ELISA, WB

    Description

    • Product name

      Recombinant Human GAD65 protein
      See all GAD65 proteins and peptides

    • Expression system

      Wheat germ

    • Accession

      Q05329

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MASPGSGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYG DAEKPAESGGSQPPRAAARKAACAC DQKPCSCSKVDVNYAFLHATDLL PACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWE LA DQPQNLEEILMHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWL TSTANTNMFTYEIAPVFVLLEYVTLKKMR EIIGWPGGSGDGIFSPGGA ISNMYAMMIARFKMFPEVKEKGMAALPRLIAFTSEHSHFSLKKGAAALGI GTDSVI LIKCDERGKMIPSDLERRILEAKQKGFVPFLVSATAGTTVYG AFDPLLAVADICKKYKIWMHVDAAWGGGLLMSR KHKWKLSGVERANSV TWNPHKMMGVPLQCSALLVREEGLMQNCNQMHASYLFQQDKHYDLSYDTG DKALQCGRHV DVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNR EGYEMVFDGKPQHTNVCFWYIPPSLRTLEDNEERMSR LSKVAPVIKAR MMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL

      • Predicted molecular weight

        90 kDa including tags

      • Amino acids

        1 to 585

    Specifications

    Our Abpromise guarantee covers the use of ab114208 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      ELISA

      Western blot

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.3% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • 65 kDa glutamic acid decarboxylase
      • DCE 2
      • DCE2
      • DCE2_HUMAN
      • GAD 2
      • GAD 65
      • GAD-2
      • GAD-65
      • Gad2
      • Glutamate decarboxylase 2
      • Glutamate decarboxylase 2 (pancreas)
      • Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa)
      • Glutamate Decarboxylase 65
      • Glutamate decarboxylase 65 kDa isoform
      • Glutamic Acid Decarboxylase 2
      • Glutamic Acid Decarboxylase 65
      • MGC161605
      • MGC161607
      see all

    • Function

      Catalyzes the production of GABA.

    • Sequence similarities

      Belongs to the group II decarboxylase family.

    • Post-translational
      modifications

      Phosphorylated; which does not affect kinetic parameters or subcellular location.
      Palmitoylated; which is required for presynaptic clustering.

    • Cellular localization

      Cytoplasm > cytosol. Cytoplasmic vesicle. Cell junction > synapse > presynaptic cell membrane. Golgi apparatus membrane. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human GAD65 protein (ab114208)
      SDS-PAGE - Recombinant Human GAD65 protein (ab114208)
      12.5% SDS-PAGE analysis of ab114208 stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab114208? Please let us know so that we can cite the reference in this datasheet.

    ab114208 has been referenced in 1 publication.

    • Fan Y  et al. Thymus-specific deletion of insulin induces autoimmune diabetes. EMBO J 28:2812-24 (2009). PubMed: 19680229

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