Recombinant Human GAD65 protein (ab114208)
Key features and details
- Expression system: Wheat germ
- Suitable for: SDS-PAGE, ELISA, WB
Description
-
Product name
Recombinant Human GAD65 protein
See all GAD65 proteins and peptides -
Expression system
Wheat germ -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MASPGSGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYG DAEKPAESGGSQPPRAAARKAACAC DQKPCSCSKVDVNYAFLHATDLL PACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWE LA DQPQNLEEILMHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWL TSTANTNMFTYEIAPVFVLLEYVTLKKMR EIIGWPGGSGDGIFSPGGA ISNMYAMMIARFKMFPEVKEKGMAALPRLIAFTSEHSHFSLKKGAAALGI GTDSVI LIKCDERGKMIPSDLERRILEAKQKGFVPFLVSATAGTTVYG AFDPLLAVADICKKYKIWMHVDAAWGGGLLMSR KHKWKLSGVERANSV TWNPHKMMGVPLQCSALLVREEGLMQNCNQMHASYLFQQDKHYDLSYDTG DKALQCGRHV DVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNR EGYEMVFDGKPQHTNVCFWYIPPSLRTLEDNEERMSR LSKVAPVIKAR MMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL -
Predicted molecular weight
90 kDa including tags -
Amino acids
1 to 585
-
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab114208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
ELISA
Western blot
-
Form
Liquid -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.3% Glutathione, 0.79% Tris HCl
General Info
-
Alternative names
- 65 kDa glutamic acid decarboxylase
- DCE 2
- DCE2
see all -
Function
Catalyzes the production of GABA. -
Sequence similarities
Belongs to the group II decarboxylase family. -
Post-translational
modificationsPhosphorylated; which does not affect kinetic parameters or subcellular location.
Palmitoylated; which is required for presynaptic clustering. -
Cellular localization
Cytoplasm > cytosol. Cytoplasmic vesicle. Cell junction > synapse > presynaptic cell membrane. Golgi apparatus membrane. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters. - Information by UniProt
Images
-
SDS-PAGE - Recombinant Human GAD65 protein (ab114208)12.5% SDS-PAGE analysis of ab114208 stained with Coomassie Blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (1)
ab114208 has been referenced in 1 publication.
- Fan Y et al. Thymus-specific deletion of insulin induces autoimmune diabetes. EMBO J 28:2812-24 (2009). PubMed: 19680229
