Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 5.000 Eu/ml
- Active: Yes
- Suitable for: WB, SDS-PAGE, Functional Studies
Product nameRecombinant human GAD65 protein
See all GAD65 proteins and peptides
Purity> 95 % SDS-PAGE.
Purified by SDS-PAGE and RP-HPLC.
Endotoxin level< 5.000 Eu/ml
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceMASPGSGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYG DAEKPAESGGSQPPRAAARKAACACDQKPCSCSKVDVNYAFLHATDLLPA CDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELA DQPQNLEEILMHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTA NTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYA MMIARFKMFPEVKEKGMAALPRLIAFTSEHSHFSLKKGAAALGIGTDSVI LIKCDERGKMIPSDLERRILEAKQKGFVPFLVSATAGTTVYGAFDPLLAV ADICKKYKIWMHVDAAWGGGLLMSRKHKWKLSGVERANSVTWNPHKMMGV PLQCSALLVREEGLMQNCNQ MHASYLFQQDKHYDLSYDTGDKALQCGR HVDVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNREGYEMVFDGK PQHTNVCFWYIPPSLRTLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQ PLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL
Predicted molecular weight65 kDa
Amino acids1 to 585
Additional sequence informationRecombinant human GAD65 expressed in baculoviral infection of Spodoptera frugiperda cells, purified & formulated in RPMI medium in human serum albumin (clinical grade) for use in T cell assays.
Our Abpromise guarantee covers the use of ab206646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: Human serum albumin, 0.03% DTT
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- 65 kDa glutamic acid decarboxylase
- DCE 2
FunctionCatalyzes the production of GABA.
Sequence similaritiesBelongs to the group II decarboxylase family.
modificationsPhosphorylated; which does not affect kinetic parameters or subcellular location.
Palmitoylated; which is required for presynaptic clustering.
Cellular localizationCytoplasm > cytosol. Cytoplasmic vesicle. Cell junction > synapse > presynaptic cell membrane. Golgi apparatus membrane. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
- Information by UniProt
SDS Page Gel analysis comparing different protein molecular weights, lanes 1 & 2 contain rhGAD65 ab206647 at concentrations of 1 µL & 0.1 µL respectively. This protein was compared to BSA (lane 3 - 1 µg & lane 4 - 10 µg) and T-cell GAD65 (ab206646) (lane 5 - 1 µL & lane 6 - 0.1 µL). The bands were run on a NuPAGE 7% Tris-Acetate Gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab206646 has not yet been referenced specifically in any publications.