Recombinant Human GALE protein (ab96767)

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SDS-PAGE - Recombinant Human GALE protein (ab96767)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: MS, SDS-PAGE

    Description

    • Product name

      Recombinant Human GALE protein
      See all GALE proteins and peptides

    • Purity

      > 95 % SDS-PAGE.
      ab96767 is purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      Q14376

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHHSSGLVPRGSHMAEKVLVTGGAGYIGSHTVLELLEAGYLPV VIDNFHNAFRGGGSLPESLRRVQELTGRSVEFEEMDILDQGALQRLFKKY SFMAVIHFAGLKAVGESVQKPLDYYRVNLTGTIQLLEIMKAHGVKNLVFS SSATVYGNPQYLPLDEAHPTGGCTNPYGKSKFFIEEMIRDLCQADKTWNA VLLRYFNPTGAHASGCIGEDPQGIPNNLMPYVSQVAIGRREALNVFGNDY DTEDGTGVRDYIHVVDLAKGHIAALRKLKEQCGCRIYNLGTGTGYSVLQM VQAMEKASGKKIPYKVVARREGDVAACYANPSLAQEELGWTAALGLDRMC EDLWRWQKQNPSGFGTQA

      • Predicted molecular weight

        40 kDa including tags

      • Amino acids

        1 to 348

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab96767 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Mass Spectrometry

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.077% DTT, 0.316% Tris HCl, 0.0292% EDTA, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

    General Info

    • Alternative names

      • FLJ95174
      • FLJ97302
      • Galactose 4 epimerase UDP
      • Galactowaldenase
      • galE
      • GALE_HUMAN
      • OTTHUMP00000002991
      • OTTHUMP00000002994
      • OTTHUMP00000037931
      • OTTHUMP00000044857
      • SDR1E1
      • short chain dehydrogenase/reductase family 1E member 1
      • UDP galactose 4 epimerase
      • UDP galactose 4' epimerase
      • UDP glucose 4 epimerase
      • UDP-galactose 4-epimerase
      • UDP-glucose 4-epimerase
      see all

    • Function

      Catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine.

    • Pathway

      Carbohydrate metabolism; galactose metabolism.

    • Involvement in disease

      Defects in GALE are the cause of epimerase-deficiency galactosemia (EDG) [MIM:230350]; also known as galactosemia type 3. Clinical features include early-onset cataracts, liver damage, deafness and mental retardation. There are two clinically distinct forms of EDG. (1) A benign, or 'peripheral' form with no detectable GALE activity in red blood cells and characterized by mild symptoms. Some patients may suffer no symptoms beyond raised levels of galactose-1-phosphate in the blood. (2) A much rarer 'generalized' form with undetectable levels of GALE activity in all tissues and resulting in severe features such as restricted growth and mental development.

    • Sequence similarities

      Belongs to the sugar epimerase family.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human GALE protein (ab96767)
      SDS-PAGE - Recombinant Human GALE protein (ab96767)
      15% SDS-PAGE showing ab96767 at approximately 40.4kDa (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab96767? Please let us know so that we can cite the reference in this datasheet.

    ab96767 has not yet been referenced specifically in any publications.

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