Recombinant human Galectin 3 protein (ab78583)

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Description

  • Product name

    Recombinant human Galectin 3 protein
    See all Galectin 3 proteins and peptides

  • Biological activity

    The activity is determined by the ability to induce chemotaxis of human PBMCs at concentrations ranging from 2-220 μg/mL. Corresponding to a specific activity of 5 x 102 units/mg.

  • Purity

    > 95 % SDS-PAGE.
    Purity Approximately 97% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. Endotoxin level as measured by LAL is <0.01ng/µg or <0.1EU/µg.

  • Expression system

    Escherichia coli

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYHGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSAPGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI

    • Predicted molecular weight

      26 kDa

    • Amino acids

      1 to 250

Specifications

Our Abpromise guarantee covers the use of ab78583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised

  • Additional notes

    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.164% Sodium phosphate, 0.29% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with sterile water at 0.1-1.0 mg/ml, which can then be further diluted to other aqueous solutions.

General Info

  • Alternative names

    • 35 kDa lectin
    • Carbohydrate binding protein 35
    • Carbohydrate-binding protein 35
    • CBP 35
    • CBP35
    • Gal-3
    • GAL3
    • Galactose-specific lectin 3
    • Galactoside-binding protein
    • GALBP
    • Galectin 3 internal gene,included
    • Galectin-3
    • Galectin3
    • GALIG
    • GBP
    • IgE binding protein
    • IgE-binding protein
    • L 31
    • L 34
    • L-31
    • L-34 galactoside-binding lectin
    • L31
    • Laminin-binding protein
    • Lectin L-29
    • Lectin, galactose binding, soluble 3
    • LEG3_HUMAN
    • LGALS2
    • LGALS3
    • MAC 2 antigen
    • Mac-2
    • Mac-2 antigen
    • MAC2
    • Macrophage galactose-specific lectin
    • MGC105387
    see all

  • Function

    Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.

  • Tissue specificity

    A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.

  • Sequence similarities

    Contains 1 galectin domain.

  • Cellular localization

    Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
  • Information by UniProt

Images

  • Other - Recombinant human Galectin 3 protein (ab78583)
    Other - Recombinant human Galectin 3 protein (ab78583)
    ab78583 used in Western Blot. Figure: 1 ug under (+) reducing and (-) non-reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Human Galectin-3 has a predicted MW of 26 kDa.

References

ab78583 has not yet been referenced specifically in any publications.

Publishing research using ab78583? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

  I would like to use your Anti-Galectin-3 antibody (ab2785) in Serum samples to detect Galectin 3 by ELISA.   I wonder if you have any concentration range that I can start with because the data sheet doesn't specific anyone.   Thank you so much.  

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Answer

Thank you for contacting us.

We recommend a starting concentration of 0.1ug/ml when using purified antibodies in ELISA. This will have to optimized for your specific protocol.

Should you need a positive control for your ELISA we recommend and offer the activeRecombinant Full length Human Galectin 3 protein, ab78583 9https://www.abcam.com/Galectin-3-protein-Active-ab78583.html).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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