Recombinant human Galectin 3 protein (ab78583)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant human Galectin 3 protein
See all Galectin 3 proteins and peptides -
Biological activity
The activity is determined by the ability to induce chemotaxis of human PBMCs at concentrations ranging from 2-220 μg/mL. Corresponding to a specific activity of 5 x 102 units/mg.
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Purity
> 95 % SDS-PAGE.
Purity Approximately 97% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. Endotoxin level as measured by LAL is <0.01ng/µg or <0.1EU/µg. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYHGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSAPGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI -
Predicted molecular weight
26 kDa -
Amino acids
1 to 250
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab78583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Additional notes
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 0.164% Sodium phosphate, 0.29% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile water at 0.1-1.0 mg/ml, which can then be further diluted to other aqueous solutions.
General Info
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Alternative names
- 35 kDa lectin
- Carbohydrate binding protein 35
- Carbohydrate-binding protein 35
see all -
Function
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. -
Tissue specificity
A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. -
Sequence similarities
Contains 1 galectin domain. -
Cellular localization
Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab78583 has not yet been referenced specifically in any publications.