Recombinant human Galectin 3 protein (ab78583)

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Western blot - Recombinant human Galectin 3 protein (ab78583)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Active: Yes
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant human Galectin 3 protein
      See all Galectin 3 proteins and peptides

    • Biological activity

      The activity is determined by the ability to induce chemotaxis of human PBMCs at concentrations ranging from 2-220 μg/mL. Corresponding to a specific activity of 5 x 102 units/mg.

    • Purity

      > 95 % SDS-PAGE.
      Purity Approximately 97% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. Endotoxin level as measured by LAL is <0.01ng/µg or <0.1EU/µg.

    • Expression system

      Escherichia coli

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYHGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSAPGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI

      • Predicted molecular weight

        26 kDa

      • Amino acids

        1 to 250

    Specifications

    Our Abpromise guarantee covers the use of ab78583 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized

    • Additional notes

      For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      Constituents: 0.164% Sodium phosphate, 0.29% Sodium chloride

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Reconstitute with sterile water at 0.1-1.0 mg/ml, which can then be further diluted to other aqueous solutions.

    General Info

    • Alternative names

      • 35 kDa lectin
      • Carbohydrate binding protein 35
      • Carbohydrate-binding protein 35
      • CBP 35
      • CBP35
      • Gal-3
      • GAL3
      • Galactose-specific lectin 3
      • Galactoside-binding protein
      • GALBP
      • Galectin 3 internal gene,included
      • Galectin-3
      • Galectin3
      • GALIG
      • GBP
      • IgE binding protein
      • IgE-binding protein
      • L 31
      • L 34
      • L-31
      • L-34 galactoside-binding lectin
      • L31
      • Laminin-binding protein
      • Lectin L-29
      • Lectin, galactose binding, soluble 3
      • LEG3_HUMAN
      • LGALS2
      • LGALS3
      • MAC 2 antigen
      • Mac-2
      • Mac-2 antigen
      • MAC2
      • Macrophage galactose-specific lectin
      • MGC105387
      see all

    • Function

      Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.

    • Tissue specificity

      A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.

    • Sequence similarities

      Contains 1 galectin domain.

    • Cellular localization

      Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
    • Information by UniProt

    Images

    • Western blot - Recombinant human Galectin 3 protein (ab78583)
      Western blot - Recombinant human Galectin 3 protein (ab78583)
      ab78583 used in Western Blot. Figure: 1 ug under (+) reducing and (-) non-reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Human Galectin-3 has a predicted MW of 26 kDa.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab78583? Please let us know so that we can cite the reference in this datasheet.

    ab78583 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

      I would like to use your Anti-Galectin-3 antibody (ab2785) in Serum samples to detect Galectin 3 by ELISA.   I wonder if you have any concentration range that I can start with because the data sheet doesn't specific anyone.   Thank you so much.  

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    Answer

    Thank you for contacting us.

    We recommend a starting concentration of 0.1ug/ml when using purified antibodies in ELISA. This will have to optimized for your specific protocol.

    Should you need a positive control for your ELISA we recommend and offer the activeRecombinant Full length Human Galectin 3 protein, ab78583 9https://www.abcam.com/Galectin-3-protein-Active-ab78583.html).

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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