Recombinant Human gamma Synuclein/SNCG protein (ab48712)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: WB, SDS-PAGE
Description
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Product name
Recombinant Human gamma Synuclein/SNCG protein
See all gamma Synuclein/SNCG proteins and peptides -
Purity
> 95 % SDS-PAGE.
The protein coding region of ß-Synuclein was amplified by RT-PCR and cloned into an E.coli expression vector. ß-Synuclein was overexpressed in E. coli and purified to apparent homogeneity by taking advantage of the thermosolubility of the protein and by using conventional column chromatography techniques. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MDVFKKGFSI AKEGVVGAVE KTKQGVTEAA EKTKEGVMYV GAKTKENVVQ SVTSVAEKTK EQANAVSEAV VSSVNTVATK TVEEAENIAV TSGVVRKEDL RPSAPQQEGV ASKEKEEVAE EAQSGGD -
Amino acids
1 to 127
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Specifications
Our Abpromise guarantee covers the use of ab48712 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.316% Tris HCl, 0.58% Sodium chloride
General Info
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Alternative names
- BCSG1
- Breast cancer specific gene 1 protein
- Breast cancer specific protein 1
see all -
Function
Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway. -
Tissue specificity
Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung. -
Sequence similarities
Belongs to the synuclein family. -
Post-translational
modificationsPhosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases. -
Cellular localization
Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle. - Information by UniProt
Images
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15% SDS-PAGE gel loaded with recombinant gamma Synuclein/SNCG protein.
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Anti-gamma Synuclein/SNCG antibody (ab46201) at 0.5 µg/ml +
Recombinant Human gamma Synuclein/SNCG protein (ab48712) at 0.05 µg
Secondary
Goat polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 13 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (3)
ab48712 has been referenced in 3 publications.
- Le T et al. Chimera RNA interference knockdown of ?-synuclein in human cortical astrocytes results in mitotic catastrophe. Neural Regen Res 15:1894-1902 (2020). WB ; Human . PubMed: 32246638
- Winham CL et al. ?-Synuclein Induces Human Cortical Astrocyte Proliferation and Subsequent BDNF Expression and Release. Neuroscience N/A:N/A (2019). PubMed: 31078687
- Surgucheva I et al. ?-Synuclein: seeding of a-synuclein aggregation and transmission between cells. Biochemistry 51:4743-54 (2012). PubMed: 22620680