Description

  • Product name

    Recombinant Human gamma Synuclein/SNCG protein
    See all gamma Synuclein/SNCG proteins and peptides
  • Purity

    > 95 % SDS-PAGE.
    The protein coding region of ß-Synuclein was amplified by RT-PCR and cloned into an E.coli expression vector. ß-Synuclein was overexpressed in E. coli and purified to apparent homogeneity by taking advantage of the thermosolubility of the protein and by using conventional column chromatography techniques.
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MDVFKKGFSI AKEGVVGAVE KTKQGVTEAA EKTKEGVMYV GAKTKENVVQ SVTSVAEKTK EQANAVSEAV VSSVNTVATK TVEEAENIAV TSGVVRKEDL RPSAPQQEGV ASKEKEEVAE EAQSGGD
    • Amino acids

      1 to 127

Associated products

Specifications

Our Abpromise guarantee covers the use of ab48712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.316% Tris HCl, 0.58% Sodium chloride

General Info

  • Alternative names

    • BCSG1
    • Breast cancer specific gene 1 protein
    • Breast cancer specific protein 1
    • Breast cancer-specific gene 1 protein
    • Gamma synuclein
    • Gamma-synuclein
    • Persyn
    • PRSN
    • SNCG
    • SR
    • Synoretin
    • Synuclein gamma
    • synuclein, gamma (breast cancer-specific protein 1)
    • SYUG_HUMAN
    see all
  • Function

    Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway.
  • Tissue specificity

    Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung.
  • Sequence similarities

    Belongs to the synuclein family.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases.
  • Cellular localization

    Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle.
  • Information by UniProt

Images

  • 15% SDS-PAGE gel loaded with recombinant gamma Synuclein/SNCG protein.

  • Anti-gamma Synuclein/SNCG antibody (ab46201) at 0.5 µg/ml + Recombinant Human gamma Synuclein/SNCG protein (ab48712) at 0.05 µg

    Secondary
    Goat polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 13 kDa

References

This product has been referenced in:

  • Winham CL  et al. ?-Synuclein Induces Human Cortical Astrocyte Proliferation and Subsequent BDNF Expression and Release. Neuroscience N/A:N/A (2019). Read more (PubMed: 31078687) »
  • Surgucheva I  et al. ?-Synuclein: seeding of a-synuclein aggregation and transmission between cells. Biochemistry 51:4743-54 (2012). Read more (PubMed: 22620680) »
See all 2 Publications for this product

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