Recombinant Human gamma Tubulin protein (denatured) (ab115710)

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Description

  • Product name

    Recombinant Human gamma Tubulin protein (denatured)

  • Purity

    > 85 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Accession

    P23258

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMPREIITLQLGQCGNQIGFEFWKQLCAEHG ISPEGIVEEFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVIHSILNS PYAKLYNPENIYLSEHGGGAGNNWASGFSQGEKIHEDIFDIIDREADGSD SLEGFVLCHSIAGGTGSGLGSYLLERLNDRYPKKLVQTYSVFPNQDEMSD VVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQ LVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQ SVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDP TQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLPSAHRVSGLMMAN HTSISSLFERTCRQYDKLRKREAFLEQFRKEDMFKDNFDEMDTSREIVQQ LIDEYHAATRPDYISWGTQEQ

    • Predicted molecular weight

      53 kDa including tags

    • Amino acids

      1 to 451

    • Tags

      His tag N-Terminus

  • Description

    Recombinant Human gamma Tubulin protein

Specifications

Our Abpromise guarantee covers the use of ab115710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 6% Urea, 0.32% Tris HCl, 5% Glycerol

General Info

  • Alternative names

    • Gamma 1 tubulin
    • Gamma 2 tubulin
    • Gamma Tubulin 1
    • Gamma Tubulin 2
    • Gamma tubulin complex component 1
    • Gamma-2-tubulin
    • GCP 1
    • GCP-1
    • GCP1
    • MGC131994
    • TBG2_HUMAN
    • TUBG
    • TUBG1
    • TUBG2
    • TUBGCP1
    • Tubulin gamma 1 chain
    • Tubulin gamma 2 chain
    • Tubulin gamma complex-associated protein 1
    • Tubulin gamma-2 chain
    • tubulin, gamma 1
    • tubulin, gamma 2
    • tubulin, gamma polypeptide
    • Xgam
    see all

  • Function

    Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.

  • Sequence similarities

    Belongs to the tubulin family.

  • Post-translational
    modifications

    Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.

  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human gamma Tubulin protein (denatured) (ab115710)
    SDS-PAGE - Recombinant Human gamma Tubulin protein (denatured) (ab115710)
    15% SDS-PAGE showing ab115710 at approximately 53.3kDa (3µg).

References

This product has been referenced in:

  • Larsson VJ  et al. Mitotic spindle assembly and ?-tubulin localisation depend on the integral nuclear membrane protein Samp1. J Cell Sci 131:N/A (2018). Read more (PubMed: 29514856) »
See 1 Publication for this product

Publishing research using ab115710? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

I Have recently purchase the above product. I have noticed on a gel there are 3 contaminates above 100KDa. Do you know what these are? They seem to be reacting with a anti gamma tubulin antibody (from another supplier). I am thinking they are aggregates but wondered if you have investigated this.

Thanks

Read More
Answer

Thank you for your enquiry and your interest in our products.

The Lab has retested this protein and it is very likely that the higher band at 100 kDa represents the multimer forms. I have attached the image to this response and hope that you can open this file. As you can see, without DTT in the SDS sample buffer, the protein didn’t move properly through the gel matrix. With the DTT, longer boiling time yielded better results i.e. less of the multimer bands and a stronger 53 kDa band.

I hope this helps and if I can assist further, please do not hesitate to contact me.

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Question

I Have recently purchase the above product. I have noticed on a gel there are 3 contaminates above 100KDa. Do you know what these are? They seem to be reacting with a anti gamma tubulin antibody (from another supplier). I am thinking they are aggregates but wondered if you have investigated this.



Thanks

Read More
Answer

I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

Please could you provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could provide the batch number and attach an image to the response.

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

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