Recombinant human GAPDH protein (Active) (ab82633)

Submit a review Q&A (4)References (4)

Description

  • Product name

    Recombinant human GAPDH protein (Active)
    See all GAPDH proteins and peptides

  • Biological activity

    Up to 30U/mg.

    One unit of activity of GAPDH is defined as the amount of enzyme required to convert 1µmole of NAD to NADH per min under standard assay conditions.

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Accession

    P04406

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE

    • Predicted molecular weight

      36 kDa

    • Amino acids

      1 to 335

Specifications

Our Abpromise guarantee covers the use of ab82633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituent: 0.476% HEPES

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with HEPES or Tris buffer or with 260 ul of distilled water. Add 10% glycerol for longer term storage.

General Info

  • Alternative names

    • 38 kDa BFA-dependent ADP-ribosylation substrate
    • aging associated gene 9 protein
    • Aging-associated gene 9 protein
    • BARS-38
    • cb609
    • EC 1.2.1.12
    • Epididymis secretory sperm binding protein Li 162eP
    • G3P_HUMAN
    • G3PD
    • G3PDH
    • GAPD
    • GAPDH
    • Glyceraldehyde 3 phosphate dehydrogenase
    • glyceraldehyde 3-PDH
    • Glyceraldehyde-3-phosphate dehydrogenase
    • HEL-S-162eP
    • KNC-NDS6
    • MGC102544
    • MGC102546
    • MGC103190
    • MGC103191
    • MGC105239
    • MGC127711
    • MGC88685
    • OCAS, p38 component
    • OCT1 coactivator in S phase, 38-KD component
    • peptidyl cysteine S nitrosylase GAPDH
    • Peptidyl-cysteine S-nitrosylase GAPDH
    • wu:fb33a10
    see all

  • Function

    Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

  • Sequence similarities

    Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

  • Post-translational
    modifications

    S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.

  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human GAPDH protein (ab82633)
    SDS-PAGE - Recombinant human GAPDH protein (ab82633)
    ab82633 (1µg) on SDS-PAGE.
  • Western blot - Recombinant human GAPDH protein (ab82633)
    Western blot - Recombinant human GAPDH protein (ab82633)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (4)

Publishing research using ab82633? Please let us know so that we can cite the reference in this datasheet.

ab82633 has been referenced in 4 publications.

  • Rambacher KM & Moniri NH Cysteine redox state regulates human ß2-adrenergic receptor binding and function. Sci Rep 10:2934 (2020). PubMed: 32076070
  • Deng J  et al. Long non-coding RNA OIP5-AS1 functions as an oncogene in lung adenocarcinoma through targeting miR-448/Bcl-2. Biomed Pharmacother 98:102-110 (2018). WB ; Human . PubMed: 29247949
  • Chen FK  et al. Acute progressive paravascular placoid neuroretinopathy with negative-type electroretinography in paraneoplastic retinopathy. Doc Ophthalmol 134:227-235 (2017). PubMed: 28382556
  • Liu TY  et al. Time-Resolved Proteomics Extends Ribosome Profiling-Based Measurements of Protein Synthesis Dynamics. Cell Syst 4:636-644.e9 (2017). PubMed: 28578850

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Question

The datasheet states the protein amount is 500ug and should be diluted in 260ul of either HEPES,TRIS or distilled water. Could you please specify regarding desired bufferpH and concentrations and what are the considerations for choosing a buffer?

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Answer



We would recommend reconstituting the GAPDH protein (Active), ab82633, with 260 uL of distilled sterilised water. This will produce a buffer of 20mM HEPES, pH 7.0. 10% glycerol can be added if longer term storage is required.
260µl is the volume used when the protein is aliquoted before being lyophilised. Using 260µl will bring the protein concentrationto 2 mg/ml.

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Question

How was this GAPDH protein purified? Is there a tag on it?

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Answer

I have checked with the lab and they have confirmed that this protein is not tagged. It was purified by ion exchange.

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Question

I would like to submit an enquiry for the product GAPDH protein (Active) (ab82633).With regards to the reconstitution of the product, it is recommended to reconstitute with HEPES or Tris buffer. Could you provide theappropriate molarity and pH of the reconstitution buffer, preferably Tris?

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Answer

We would recommend reconstituting the GAPDH protein (Active), ab82633, with 260 uL of distilled sterilised water. This will produce a buffer of 20mM HEPES, pH 7.4. 10% glycerol can be added if longer term storage is required.

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Question

I would like to enquire the difference between these two products:

GAPDH protein (active) (ab82633)

GAPDH protein (human) (ab77109)

I am looking for an active GAPDH protein. Please advise which is suitable.

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Answer

The GAPDH protein ab82633 and ab77109 are both the full length GAPDH expressed in E. coli, however only ab82633 has been tested for its enzymatic activity. The specific activity was found to be 35.7 U/mg. One unit of activity of GAPDH is defined as the amount of enzyme required to convert 1 µmole of NAD to NADH per minute under standard assay conditions.

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