Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant Human GATA3 protein
Purity> 90 % SDS-PAGE.
refolded and chromatographically purified.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMASMTGGQQMGRGHHHHHHENLYFQGEVTADQPRWVSHHHPAVLNGQHPD THHPGLSHSYMDAA QYPLPEEVDVLFNIDGQGNHVPPYYGNSVRATVQ RYPPTHHGSQVCRPPLLHGSLPWLDGGKAL GSHHTASPWNLSPFSKTS IHHGSPGPLSVYPPASSSSLSGGHASPHLFTFPPTPPKDVSPDPSL ST PGSAGSARQDEKECLKYQVPLPDSMKLESSHSRGSMTALGGASSSTHHPI TTYPPYVPEYSS GLFPPSSLLGGSPTGFGCKSRPKARSSTGRECVNCG ATSTPLWRRDGTGHYLCNACGLYHKMNG QNRPLIKPKRRLSAARRAGT SCANCQTTTTTLWRRNANGDPVCNACGLYYKLHNINRPLTMKKE GIQT RNRKMSSKSKKCKKVHDSLEDFPKNSSFNPAALSRHMSSLSHISPFSHSS HMLTTPTPMH PPSSLSFGPHHPSSMVTAMG
Predicted molecular weight51 kDa including tags
Amino acids2 to 443
Additional sequence informationFull length protein without initiator methionine and a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal
- Anti-GATA3 antibody (ab106625)
- Anti-TEV Tag antibody [KT88] (ab179875)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-6X His tag® antibody (ab9108)
- Anti-T7 tag® antibody (ab9115)
- Anti-T7 tag® antibody (ab9138)
Our Abpromise guarantee covers the use of ab204202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.32% Tris HCl, Sodium chloride, Potassium chloride, L-Arginine, EDTA, Glycerol
Also contains DTT.
- GATA 3
- GATA binding factor 3
- GATA binding protein 3
FunctionTranscriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
Tissue specificityT-cells and endothelial cells.
Involvement in diseaseDefects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
Sequence similaritiesContains 2 GATA-type zinc fingers.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab204202 has not yet been referenced specifically in any publications.