Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human GDF11 protein (Active)
See all GDF11 proteins and peptides
Alkaline phosphatase activity induced in ATDC-5 cells.
Acceptance Criteria: ED50 ≤100 ng/mL(≥ 1.0 x 104 units/mg).
Purity> 95 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceNLGLDCDEHSSESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGQCEYM FMQKYPHTHLVQQANPRGSAGPCCTPTKMSPINMLYFNDKQQIIYGKIPG MVVDRCGCS
Predicted molecular weight13 kDa
Amino acids299 to 407
Additional sequence informationSource: genetically modified E.coli. Mature protein without the signal peptide and propeptide Exists as a dimer. Predicted MW: Dimer, 12.5/24.9 kDa (with 109/218 amino acids)
Our Abpromise guarantee covers the use of ab218080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product shares 100% homology with amino acids 297-405 of Rat and Mouse GDF11.
This product was previously labelled as BMP11, BMP11 + GDF8 / Myostatin
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C.
Constituent: 0.1% Trifluoroacetic acid
Lyophilized from a sterile (0.2 micron) filtered aqueous solution.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionSterile 10 mM HCl at 0.1 mg/mL. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
- BMP 11
FunctionSecreted signal that acts globally to specify positional identity along the anterior/posterior axis during development. Play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern.
Sequence similaritiesBelongs to the TGF-beta family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab218080 has not yet been referenced specifically in any publications.