Bioactive grade

Recombinant human GDF11 protein (Animal Free) (ab218103)

Description

  • Product name

    Recombinant human GDF11 protein (Animal Free)
    See all GDF11 proteins and peptides
  • Biological activity

    Alkaline phosphatase activity in ATDC5 cells: ED50 ≤100 ng/ml; ≥ 1.0 x 104 units/mg (typical ED50 is < 10 ng/mL).

  • Purity

    > 95 % SDS-PAGE.
    Reducing and non-reducing SDS-PAGE.
  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    Yes
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      NLGLDCDEHS SESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGQCEYM FMQKYPHTHL VQQANPRGSA GPCCTPTKMS PINMLYFNDK QQIIYGKIPG MVVDRCGCS
    • Predicted molecular weight

      13 kDa
    • Amino acids

      299 to 407
    • Additional sequence information

      This product is for the mature full length protein. The signal peptide and propeptide are not included.

Specifications

Our Abpromise guarantee covers the use of ab218103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    HPLC

  • Form

    Lyophilised
  • Additional notes

    This product shares 100% homology with amino acids 297-405 of Rat and Mouse GDF11.

    This product was previously labelled as BMP11, BMP11 + GDF8 / Myostatin

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at Room Temperature. Store at -20°C or -80°C.

    Constituent: 0.1% Trifluoroacetic acid

    0.2 micron filtered

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute in sterile 10mM HCL at 0.1 mg/mL. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

General Info

  • Alternative names

    • BMP 11
    • BMP-11
    • BMP11
    • Bone morphogenetic protein 11
    • GDF 11
    • GDF-11
    • Gdf11
    • GDF11_HUMAN
    • Growth differentiation factor 11
    • Growth/differentiation factor 11
    see all
  • Function

    Secreted signal that acts globally to specify positional identity along the anterior/posterior axis during development. Play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern.
  • Sequence similarities

    Belongs to the TGF-beta family.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • Functional analysis of ab218103
  • SDS-PAGE analysis of ab218103 (1μg) stained with Coomassie blue:

    Lane 1: Non-reduced

    Lane 2: Reduced

    4-20% Tris-Glycine gel

References

ab218103 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab218103.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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