Recombinant human GDF15 protein (ab125769)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human GDF15 protein
See all GDF15 proteins and peptides -
Biological activity
ab125769 is a non-glycosylated, disulfide linked homodimer. It is comprised of two identical 120 amino acid monomers. The activity is determined by the inhibition of DU-145 cells and is typically 1-2 (μg/mL).
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 0.100 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MARNGDHCPL GPGRCCRLHT VRASLEDLGW ADWVLSPREV QVTMCIGACP SQFRAANMHA QIKTSLHRLK PDTVPAPCCV PASYNPMVLI QKTDTGVSLQ TYDDLLAKDC HCI -
Amino acids
197 to 308 -
Additional sequence information
This product refers to the processed mature form of this protein from aa 195 to 308. The signal peptide and propeptide are removed.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab125769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionCentrifuge vial before opening. It is recommended to reconstitute the lyophilized product with sterile 5 mM acetic acid at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
General Info
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Alternative names
- GDF 15
- GDF-15
- Gdf15
see all -
Tissue specificity
Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (1)
ab125769 has been referenced in 1 publication.
- Olsen OE et al. TGF-ß contamination of purified recombinant GDF15. PLoS One 12:e0187349 (2017). PubMed: 29161287
