Recombinant Human GDF8 / Myostatin protein (ab203723)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, WB, SDS-PAGE, MS
Description
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Product name
Recombinant Human GDF8 / Myostatin protein
See all GDF8 / Myostatin proteins and peptides -
Purity
> 95 % SDS-PAGE.
Assessed by densitometry. Purifed using Ni-NTA chromatography. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
HHHHHHASNENSEQKENVEKEGLCNACTWRQNTKSSRIEAIKIQILSKLR LETAPNISKDVIRQLLPKAPPLRELIDQYDVQRDDSSDGSLEDDDYHATT ETIITMPTESDFLMQVDGKPKCCFFKFSSKIQYNKVVKAQLWIYLRPVET PTTVFVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIWQSIDVKTVLQNW LKQPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVTDTPKRSR RDFGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEF VFLQKYPHTHLVHQANPRGSAGPCCTPTKMSPINMLYFNGKEQIIYGKIP AMVVDRCGCS -
Predicted molecular weight
71 kDa including tags -
Amino acids
24 to 375 -
Tags
His tag N-Terminus -
Additional sequence information
Mature protein and Propeptide without Signal peptide.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab203723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
Western blot
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
LC-MS/MS -
Form
Liquid -
Additional notes
Frozen from a 0.25–0.5 mg/ml solution. Defrost at ambient temperature. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 20% Glycerol, 80% PBS
General Info
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Alternative names
- GDF 8
- GDF-8
- GDF8
see all -
Function
Acts specifically as a negative regulator of skeletal muscle growth. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human GDF8 / Myostatin protein (ab203723)
14 % SDS-PAGE analysis of ab203723:
1. Reduced and boiled sample, 2.5 μg/lane.
2. Non-reduced and non-boiled sample, 2.5 μg/lane.The approx. 50 kDa band represents the complex of Myostatin-Propeptide. The band corresponding to approx. 38 kDa represents cleaved form of Propeptide and similarly the approx. 12 kDa band belongs to cleaved Myostatin domain. Myostatin domains makes dimmers in non-reducing conditions resulting in bends of approx. 25 and 97 kDa, respectively.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab203723 has not yet been referenced specifically in any publications.