Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, WB, SDS-PAGE, MS
Product nameRecombinant Human GDF8 / Myostatin protein
See all GDF8 / Myostatin proteins and peptides
Purity> 95 % SDS-PAGE.
Assessed by densitometry. Purifed using Ni-NTA chromatography.
Endotoxin level< 1.000 Eu/µg
Expression systemHEK 293 cells
Protein lengthFull length protein
SequenceHHHHHHASNENSEQKENVEKEGLCNACTWRQNTKSSRIEAIKIQILSKLR LETAPNISKDVIRQLLPKAPPLRELIDQYDVQRDDSSDGSLEDDDYHATT ETIITMPTESDFLMQVDGKPKCCFFKFSSKIQYNKVVKAQLWIYLRPVET PTTVFVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIWQSIDVKTVLQNW LKQPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVTDTPKRSR RDFGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEF VFLQKYPHTHLVHQANPRGSAGPCCTPTKMSPINMLYFNGKEQIIYGKIP AMVVDRCGCS
Predicted molecular weight71 kDa including tags
Amino acids24 to 375
TagsHis tag N-Terminus
Additional sequence informationMature protein and Propeptide without Signal peptide.
Our Abpromise guarantee covers the use of ab203723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Frozen from a 0.25–0.5 mg/ml solution. Defrost at ambient temperature. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 20% Glycerol, 80% PBS
- GDF 8
FunctionActs specifically as a negative regulator of skeletal muscle growth.
Sequence similaritiesBelongs to the TGF-beta family.
- Information by UniProt
14 % SDS-PAGE analysis of ab203723:
1. Reduced and boiled sample, 2.5 μg/lane.
2. Non-reduced and non-boiled sample, 2.5 μg/lane.
The approx. 50 kDa band represents the complex of Myostatin-Propeptide. The band corresponding to approx. 38 kDa represents cleaved form of Propeptide and similarly the approx. 12 kDa band belongs to cleaved Myostatin domain. Myostatin domains makes dimmers in non-reducing conditions resulting in bends of approx. 25 and 97 kDa, respectively.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab203723 has not yet been referenced specifically in any publications.