Recombinant Human GDF8 / Myostatin protein (ab203723)
- Datasheet
- References
- Protocols
Description
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Product name
Recombinant Human GDF8 / Myostatin protein
See all GDF8 / Myostatin proteins and peptides -
Purity
> 95 % SDS-PAGE.
Assessed by densitometry. Purifed using Ni-NTA chromatography. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
HHHHHHASNENSEQKENVEKEGLCNACTWRQNTKSSRIEAIKIQILSKLR LETAPNISKDVIRQLLPKAPPLRELIDQYDVQRDDSSDGSLEDDDYHATT ETIITMPTESDFLMQVDGKPKCCFFKFSSKIQYNKVVKAQLWIYLRPVET PTTVFVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIWQSIDVKTVLQNW LKQPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVTDTPKRSR RDFGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEF VFLQKYPHTHLVHQANPRGSAGPCCTPTKMSPINMLYFNGKEQIIYGKIP AMVVDRCGCS -
Predicted molecular weight
71 kDa including tags -
Amino acids
24 to 375 -
Tags
His tag N-Terminus -
Additional sequence information
Mature protein and Propeptide without Signal peptide.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab203723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
Western blot
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
LC-MS/MS -
Form
Liquid -
Additional notes
Frozen from a 0.25–0.5 mg/ml solution. Defrost at ambient temperature. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8
Constituents: 20% Glycerol, 80% PBS
General Info
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Alternative names
- GDF 8
- GDF-8
- GDF8
see all -
Function
Acts specifically as a negative regulator of skeletal muscle growth. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human GDF8 / Myostatin protein (ab203723)14 % SDS-PAGE analysis of ab203723:
1. Reduced and boiled sample, 2.5 μg/lane.
2. Non-reduced and non-boiled sample, 2.5 μg/lane.The approx. 50 kDa band represents the complex of Myostatin-Propeptide. The band corresponding to approx. 38 kDa represents cleaved form of Propeptide and similarly the approx. 12 kDa band belongs to cleaved Myostatin domain. Myostatin domains makes dimmers in non-reducing conditions resulting in bends of approx. 25 and 97 kDa, respectively.
Datasheets and documents
References
ab203723 has not yet been referenced specifically in any publications.
