Recombinant human GDNF protein (ab9790)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Molecular weight
      24 kDa

Associated products

Specifications

Our Abpromise guarantee covers the use of ab9790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Determined by a cell proliferation assay using SH-SY5Y cells. The expected ED50 for this effect is 1.0 - 10.0 ng/ml.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    For lot specific reconstitution information please contact our Scientific Support Team.

General Info

  • Alternative names
    • Astrocyte derived trophic factor
    • Astrocyte derived trophic factor 1
    • Astrocyte-derived trophic factor
    • Atf
    • ATF 1
    • ATF 2
    • ATF1
    • ATF2
    • gdnf
    • GDNF_HUMAN
    • Glial cell derived neurotrophic factor
    • Glial Cell Line Derived Neurotrophic Factor
    • Glial cell line-derived neurotrophic factor
    • Glial derived neurotrophic factor
    • HFB1 GDNF
    • hGDNF
    • HSCR3
    see all
  • Function
    Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
  • Tissue specificity
    In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen.
  • Involvement in disease
    Defects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
    Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.
  • Sequence similarities
    Belongs to the TGF-beta family. GDNF subfamily.
  • Cellular localization
    Secreted.
  • Information by UniProt

References

ab9790 has not yet been referenced specifically in any publications.

Publishing research using ab9790? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

We distribute your products in Russia. One of customers is after purchasing protein GDNF and has doubts which product to buy. What she is asking is primary sequence for active form of ab73450. Alternatively she’d like to get any available info on specific activity of ab9790 that has primary sequence shown on website.

Could you please assist with such an enquiry?

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Answer

Thank you for contacting us.

Here is some further information about the two products:

ab73450 - http://www.uniprot.org/uniprot/P39905for the Swiss-Prot entry P39905
ab9790 - The ED50 was determined by the proliferation of rat C6 cells is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.

Just let me know if you need further information.

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Question

send datasheet links for ab9790, ab56286, and ab73450

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Answer

Thank you for contacting us.

The information we talked about over the phone can be found on the online datasheets. Please see the links below:

ab9790: https://www.abcam.com/gdnf-protein-ab9790.html

ab56286: https://www.abcam.com/gdnf-protein-ab56286.html

ab73450: https://www.abcam.com/gdnf-protein-active-ab73450.html

Please let me know if you require any additional information, and I'd be happy to help.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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