Overview

Description

  • Nature

    Recombinant
  • Source

    Wheat germ
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      MAAAFEASGALAAVATAMPAEHVAVQVPAPEPTPGPVRILRTAQDLSSPR TRTGDVLLAEPADFESLLLSRPVLEGLRAAGFERPSPVQLKAIPLGRCGL DLIVQAKSGTGKTCVFSTIALDSLVLENLSTQILILAPTREIAVQIHSVI TAIGIKMEGLECHVFIGGTPLSQDKTRLKKCHIAVGSPGRIKQLIELDYL NPGSIRLFILDEADKLLEEGSFQEQINWIYSSLPASKQMLAVSATYPEFL ANALTKYMRDPTFVRLNSSDPSLIGLKQYYKVVNSYPLAHKVFEEKTQHL QELFSRIPFNQALVFSNLHSRAQHLADILSSKGFPAECISGNMNQNQRLD AMAKLKHFHCRVLISTDLTSRGIDAEKVNLVVNLDVPLDWETYMHRIGRA GRFGTLGLTVTYCCRGEEGNMMMRIAQKCNINLLPLPDPIPSGLMEECVD WDVEVKAAVHTYGIASVPNQPLKKQIQKIERTLQIQKAHGDHMASSRNNS VSGLSVKSKNNTKQKLPVKSHSECGIIEKATSPKELGCDRQSEEQMKNSV QTPVENSTNSQHQVKEALPVSLPQIPCLSSFKIHQPYTLTFAELVEDYEH YIKEGLEKPVEIIRHYTGPGDQTVNPQNGFVRNKVTEQRVPVLASSSQSG DSESDSDSYSSRTSSQSKGNKSYLEGSSDNQLKDSESTPVDDRISLEQPP NGSDTPNPEKYQESPGIQMKTRLKEGASQRAKQSRRNLPRRSSFRLQTEA QEDDWYDCHREIRLSFSDTYQDYEEYWRAYYRAWQEYYAAASHSYYWNAQ RHPSWMAAYHMNTIYLQEMMHSNQ
    • Molecular weight

      119 kDa including tags
    • Amino acids

      1 to 824

Specifications

Our Abpromise guarantee covers the use of ab132035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    ELISA

  • Form

    Liquid
  • Additional notes

    Protein concentration is above or equal to 0.05 µg/µl.
    Best use within three months from the date of receipt of this protein.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • Component of gems 3
    • DDX 20
    • ddx20
    • DDX20_HUMAN
    • DEAD (Asp Glu Ala Asp) box polypeptide 20
    • DEAD box protein 20
    • DEAD box protein DP 103
    • DEAD box protein DP103
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 103kD
    • DEAD/H BOX 20
    • DKFZP434H052
    • DP 103
    • DP103
    • Gemin-3
    • Gemin3
    • Probable ATP dependent RNA helicase DDX20
    • Probable ATP-dependent RNA helicase ddx20
    • SMN interacting protein
    see all
  • Function

    The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. It may also play a role in the metabolism of snoRNPs.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the DEAD box helicase family. DDX20 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization

    Cytoplasm. Nucleus > gem. Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies.
  • Information by UniProt

Images

  • 12.5% SDS-PAGE stained with Coomassie Blue showing ab132035 at approximately 118.6 kDa.

References

ab132035 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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