Description

  • Product name

    Recombinant human Germinal Center Kinase/GCK protein
  • Biological activity

    The Specific activity of ab89753 was determined to be 290 nmol/min/mg.
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab89753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

    Functional Studies

  • Form

    Liquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

     This product was previously labelled as Germinal Center Kinase

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • B lymphocyte serine/threonine protein kinase
    • B lymphocyte serine/threonine-protein kinase
    • BL44
    • GC kinase
    • GCK
    • Germinal center kinase
    • germinal centre kinase (GC kinase)
    • M4K2_HUMAN
    • Map4k2
    • MAPK/ERK kinase kinase kinase 2
    • MEK kinase kinase 2
    • MEKKK 2
    • Mitogen activated protein kinase kinase kinase kinase 2
    • Mitogen-activated protein kinase kinase kinase kinase 2
    • Rab8 interacting protein
    • Rab8 interacting protein, formerly
    • Rab8-interacting protein
    • RAB8IP
    • RAB8IP, formerly
    see all
  • Function

    Enhances MAP3K1 oligomerization, which may relieve amino-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. May play a role in the regulation of vesicle targeting or fusion.
  • Tissue specificity

    Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CNH domain.
    Contains 1 protein kinase domain.
  • Cellular localization

    Cytoplasm. Basolateral cell membrane. Golgi apparatus membrane.
  • Information by UniProt

Images

  • The specific activity of Germinal Center Kinase/GCK (ab89753) was determined to be 46.1 nmol/min/mg as per activity assay protocol and was equivalent to 246 nmol/min/mg as per radiometric assay
  • SDS PAGE analysis of ab89753
  • SDS PAGE analysis of ab89753
  • SDS-PAGE showing ab89753 at approximately 125kDa.
  • Kinase Assay demonstrating specific activity of ab89753.

References

ab89753 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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