Overview

Description

  • Nature
    Recombinant
  • Source
    Wheat germ
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MAEAEGESLESWLNKATNPSNRQEDWEYIIGFCDQINKELEGPQIAVRLL AHKIQSPQEWEALQALTYLGDRVSEKVKTKVIELLYSWTMALPEEAKIKD AYHMLKRQGIVQSDPPIPVDRTLIPSPPPRPKNPVFDDEEKSKLLAKLLK SKNPDDLQEANKLIKSMVKEDEARIQKVTKRLHTLEEVNNNVRLLSEMLL HYSQEDSSDGDRELMKELFDQCENKRRTLFKLASETEDNDNSLGDILQAS DNLSRVINSYKTIIEGQVINGEVATLTLPDSEGNSQCSNQGTLIDLAELD TTNSLSSVLAPAPTPPSSGIPILPPPPQASGPPRSRSSSQAEATLGPSST SNALSWLDEELLCLGLADPAPNVPPKESAGNSQWHLLQREQSDLDFFSPR PGTAACGASDAPLLKPSAPSSSSSQAPLPPPFPAPVVPASVPAPSAGSSL FSTGVAPALAPKVEPAVPGHHGLALGNSALHHLDALDQLLEEAKVTSGLV KPTTSPLIPTTTPARPLLPFSTGPGSPLFQPLSFQSQGSPPKGPELSLAS IHVPLESIKPSSALPVTAYDKNGFRILFHFAKECPPGRPDVLVVVVSMLN TAPLPVKSIVLQAAVPKSMKVKLQPPSGTELSPFSPIQPPAAITQVMLLA NPLKEKVRLRYKLTFALGEQLSTEVGEVDQFPPVEQWGNL
    • Amino acids
      1 to 690
    • Tags
      proprietary tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab161553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Western blot

  • Form
    Liquid
  • Additional notes
    Protein concentration is above or equal to 0.05 mg/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • ADP ribosylation factor binding protein 3
    • ADP ribosylation factor binding protein GGA 3
    • ADP ribosylation factor binding protein GGA3
    • ADP-ribosylation factor-binding protein GGA3
    • ARF binding protein GGA 3
    • ARF binding protein GGA3
    • ARF-binding protein 3
    • gamma ear-containing
    • GGA 3
    • GGA3
    • GGA3_HUMAN
    • Golgi associated gamma adaptin ear containing ARF binding protein 3
    • Golgi localized gamma ear containing ARF binding protein 3
    • Golgi-localized
    • KIAA0154
    see all
  • Function
    Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the GGA protein family.
    Contains 1 GAE domain.
    Contains 1 GAT domain.
    Contains 1 VHS domain.
  • Domain
    The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
    The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
    The unstructured hinge region contains clathrin-binding and an autoinhibitory (AC-LL) motifs.
    The GAE domain binds accessory proteins regulating GGAs function.
  • Post-translational
    modifications
    Phosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
    Ubiquitinated.
  • Cellular localization
    Golgi apparatus > trans-Golgi network membrane. Endosome membrane.
  • Information by UniProt

Images

  • ab161553 on a 12.5% SDS-PAGE stained with Coomassie Blue.

References

ab161553 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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