Giantin-GST (ab152432) was used as used as a bait for the GST-immunoprecipitation of the complexes from HeLa cells lysates

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Immunoprecipitation
To detect the complexes between N-terminal of giantin and other Golgi proteins, we employed the recombinant human N-terminal GST-giantin fusion peptide (3-92 aa). This peptide is successfully immobilized by anti-GST Ab ab19256 coupled Epoxy beads (Dynabeads M-450), according to the GST Western blot (W-B) - see picture.

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Submitted Nov 19 2018

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