Description

  • Product name

    Recombinant Human GlcAT-I protein (His tag)
    See all GlcAT-I proteins and peptides
  • Purity

    > 92 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      EALPTIYVV TPTYARLVQK AELVRLSQTL SLVPRLHWLL VEDAEGPTPL VSGLLAASGL LFTHLVVLTP KAQRLREGEP GWVHPRGVEQ RNKALDWLRG RGGAVGGEKD PPPPGTQGVV YFADDDNTYS RELFEEMRWT RGVSVWPVGL VGGLRFEGPQ VQDGRVVGFH TAWEPSRPFP VDMAGFAVAL PLLLDKPNAQ FDSTAPRGHL ESSLLSHLVD PKDLEPRAAN CTRVLVWHTR TEKPKMKQEE QLQRQGRGSD PAIEV
    • Predicted molecular weight

      30 kDa including tags
    • Amino acids

      72 to 335
    • Tags

      His tag N-Terminus
    • Additional sequence information

      (AAH07906).

Specifications

Our Abpromise guarantee covers the use of ab220557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

    Previously labelled as B3GAT3. 

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -80°C. Avoid freeze / thaw cycle. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C..

    pH: 8.00
    Constituents: 0.61% Tris, 0.87% Sodium chloride, 5% Trehalose

    Lyophilized from 0.22 µm filtered solution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 1mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • 3-Gal-R glucuronyltransferase
    • 3-glucuronyltransferase 3
    • B3GA3_HUMAN
    • B3GAT3
    • Beta 1,3 glucuronyltransferase 3
    • Beta 1,3 glucuronyltransferase 3 (glucuronosyltransferase I)
    • Beta-1
    • EC 2.4.1.135
    • Galactosylgalactosylxylosylprotein 3 beta glucuronosyltransferase 3
    • Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3
    • GlcAT-I
    • GLCATI
    • glcUAT I
    • GlcUAT-I
    • GlcUATI
    • Glucuronosyltransferase I
    • Sqv 8 like protein
    • UDP GlcUA:Gal beta 1,3 Gal R glucuronyltransferase
    • UDP GlcUA:Gal beta 1,3 GalR glucuronyltransferase
    • UDP-GlcUA:Gal beta-1
    see all
  • Function

    Glycosaminoglycans biosynthesis. Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc.
  • Tissue specificity

    Ubiquitous (but weakly expressed in all tissues examined).
  • Pathway

    Protein modification; protein glycosylation.
  • Involvement in disease

    Defects in B3GAT3 are the cause of multiple joint dislocations short stature craniofacial dysmorphism and congenital heart defects (JDSSDHD) [MIM:245600]. An autosomal recessive disease characterized by dysmorphic facies, bilateral dislocations of the elbows, hips, and knees, clubfeet, and short stature, as well as cardiovascular defects.
  • Sequence similarities

    Belongs to the glycosyltransferase 43 family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Golgi apparatus membrane. Golgi apparatus > cis-Golgi network.
  • Information by UniProt

Images

  • SDS-PAGE analysis of DDT-reduced ab220557 stained overnight with Coomassie Blue.

References

ab220557 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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