Recombinant human Glucokinase protein (ab123840)

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SDS-PAGE - Recombinant human Glucokinase protein (ab123840)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 80% SDS-PAGE
    • Active: Yes
    • Tags: DDDDK tag C-Terminus
    • Suitable for: Functional Studies, SDS-PAGE

    Description

    • Product name

      Recombinant human Glucokinase protein
      See all Glucokinase proteins and peptides

    • Biological activity

      Biological Activity: 303 pmol/min/µg. One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30oC. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 µg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30°C for 30 min.

    • Purity

      > 80 % SDS-PAGE.

    • Expression system

      Escherichia coli

    • Accession

      P35557

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MLDDRARMEAAKKEKVEQILAEFQLQEEDLKKVMRRMQKEMDRGLRLETH EEASVKMLPT YVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEEGQ WSVKTKHQMYSIPEDAMTGTAE MLFDYISECISDFLDKHQMKHKKLPL GFTFSFPVRHEDIDKGILLNWTKGFKASGAEGNN VVGLLRDAIKRRGD FEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQN VE LVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKL IGGKYMGE LVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVES DTGDRKQIYNILSTLGLRPS TTDCDIVRRACESVSTRAAHMCSAGLAG VINRMRESRSEDVMRITVGVDGSVYKLHPSFK ERFHASVRRLTPSCEI TFIESEEGSGRGAALVSAVACKKACMLGQ

      • Predicted molecular weight

        53 kDa including tags

      • Amino acids

        1 to 465

      • Tags

        DDDDK tag C-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab123840 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      SDS-PAGE

    • Form

      Liquid

    • Additional notes

      303 pmol/min/µg. One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30°C. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 µg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30°C for 30 min.
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      Constituents: 1.06% Potassium phosphate, 0.008% DTT, 0.015% EDTA, 2.5% Glycerol (glycerin, glycerine), 0.29% Sodium chloride

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • ATP:D-hexose 6-phosphotransferase
      • FGQTL3
      • GCK
      • GK
      • GLK
      • Glucokinase
      • Hexokinase D pancreatic isozyme
      • Hexokinase type IV
      • Hexokinase-4
      • Hexokinase-D
      • HHF3
      • HK IV
      • HK4
      • HKIV
      • HXK4_HUMAN
      • HXKP
      • LGLK
      • MODY2
      see all

    • Function

      Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.

    • Tissue specificity

      Isoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver.

    • Involvement in disease

      Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) [MIM:125851]; also shortened MODY-2. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
      Defects in GCK are the cause of familial hyperinsulinemic hypoglycemia type 3 (HHF3) [MIM:602485]; also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur.

    • Sequence similarities

      Belongs to the hexokinase family.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant human Glucokinase protein (ab123840)
      SDS-PAGE - Recombinant human Glucokinase protein (ab123840)
      SDS-PAGE showing ab123840 (10µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab123840? Please let us know so that we can cite the reference in this datasheet.

    ab123840 has been referenced in 1 publication.

    • Cho J  et al. L-Arginine prevents cereblon-mediated ubiquitination of glucokinase and stimulates glucose-6-phosphate production in pancreatic ß-cells. Commun Biol 3:497 (2020). PubMed: 32901087

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