Recombinant human Glutamine Synthetase protein (ab222354)

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Description

  • Product name

    Recombinant human Glutamine Synthetase protein
    See all Glutamine Synthetase proteins and peptides

  • Biological activity

    Specific activity is > 2.000 pmol/min/ug, and is defined as the amount of enzyme that convert L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH.

  • Purity

    > 85 % SDS-PAGE.
    Purified by conventional chromatography techniques.

  • Expression system

    Escherichia coli

  • Accession

    P15104

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MTTSASSHLNKGIKQVYMSLPQGEKVQAMYIWIDGTGEGLRCKTRTLDSE PKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCE VFKYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWP SNGFPGPQGPYYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPA QWEFQIGPCEGISMGDHLWVARFILHRVCEDFGVIATFDPKPIPGNWNGA GCHTNFSTKAMREENGLKYIEEAIEKLSKRHQYHIRAYDPKGGLDNARRL TGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDRRPSANCDPFS VTEALIRTCLLNETGDEPFQYKN

    • Predicted molecular weight

      42 kDa

    • Amino acids

      1 to 373

Specifications

Our Abpromise guarantee covers the use of ab222354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Mass spectrometry

    MALDI-TOF

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.02% DTT, 10% Glycerol, 0.002% PMSF, 0.32% Tris HCl

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • cell proliferation-inducing protein 59
    • Cgl2214
    • GLNA
    • GLNA_HUMAN
    • GLNS
    • GLUL
    • Glutamate ammonia ligase
    • Glutamate decarboxylase
    • Glutamate--ammonia ligase
    • glutamine synthase
    • Glutamine synthetase
    • glutamine synthetase I
    • GS
    • PIG 43
    • PIG 59
    • PIG43
    • PIG59
    • Proliferation inducing protein 43
    see all

  • Function

    This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.

  • Involvement in disease

    Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.

  • Sequence similarities

    Belongs to the glutamine synthetase family.

  • Developmental stage

    Expressed during early fetal stages.

  • Cellular localization

    Cytoplasm. Mitochondrion.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human Glutamine Synthetase protein (ab222354)
    SDS-PAGE - Recombinant human Glutamine Synthetase protein (ab222354)

    Recombinant human Glutamine synthetase (3μg) analysed by 15% SDS-PAGE.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab222354? Please let us know so that we can cite the reference in this datasheet.

ab222354 has not yet been referenced specifically in any publications.

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