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    recombinant-human-glutamine-synthetase-protein-ab222354.pdf

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Recombinant human Glutamine Synthetase protein (ab222354)

  • Datasheet
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SDS-PAGE - Recombinant human Glutamine Synthetase protein (ab222354)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 85% SDS-PAGE
    • Active: Yes
    • Suitable for: SDS-PAGE, Functional Studies

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    Description

    • Product name

      Recombinant human Glutamine Synthetase protein
      See all Glutamine Synthetase proteins and peptides
    • Biological activity

      Specific activity is > 2.000 pmol/min/ug, and is defined as the amount of enzyme that convert L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH.

    • Purity

      > 85 % SDS-PAGE.
      Purified by conventional chromatography techniques.
    • Expression system

      Escherichia coli
    • Accession

      P15104
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MTTSASSHLNKGIKQVYMSLPQGEKVQAMYIWIDGTGEGLRCKTRTLDSE PKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCE VFKYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWP SNGFPGPQGPYYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPA QWEFQIGPCEGISMGDHLWVARFILHRVCEDFGVIATFDPKPIPGNWNGA GCHTNFSTKAMREENGLKYIEEAIEKLSKRHQYHIRAYDPKGGLDNARRL TGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDRRPSANCDPFS VTEALIRTCLLNETGDEPFQYKN
      • Predicted molecular weight

        42 kDa
      • Amino acids

        1 to 373

    Associated products

    • Related Products

      • Anti-Glutamine Synthetase antibody [EPR16661] (ab197024)
      • HRP Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab199198)

    Specifications

    Our Abpromise guarantee covers the use of ab222354 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

    • Mass spectrometry

      MALDI-TOF
    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.02% DTT, 10% Glycerol (glycerin, glycerine), 0.002% PMSF, 0.32% Tris HCl

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • cell proliferation-inducing protein 59
      • Cgl2214
      • GLNA
      • GLNA_HUMAN
      • GLNS
      • GLUL
      • Glutamate ammonia ligase
      • Glutamate decarboxylase
      • Glutamate--ammonia ligase
      • glutamine synthase
      • Glutamine synthetase
      • glutamine synthetase I
      • GS
      • PIG 43
      • PIG 59
      • PIG43
      • PIG59
      • Proliferation inducing protein 43
      see all
    • Function

      This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
    • Involvement in disease

      Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
    • Sequence similarities

      Belongs to the glutamine synthetase family.
    • Developmental stage

      Expressed during early fetal stages.
    • Cellular localization

      Cytoplasm. Mitochondrion.
    • Target information above from: UniProt accession P15104 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant human Glutamine Synthetase protein (ab222354)
      SDS-PAGE - Recombinant human Glutamine Synthetase protein (ab222354)

      Recombinant human Glutamine synthetase (3μg) analysed by 15% SDS-PAGE.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab222354? Please let us know so that we can cite the reference in this datasheet.

    ab222354 has not yet been referenced specifically in any publications.

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