Recombinant Human Glutaredoxin 1 protein (His tag) (ab219880)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MAQEFVNCKIQPGKVVVFIKPTCPYCRRAQEILSQLPIKQGLLEFVDITA TNHTNEIQDYLQQLTGARTVPRVFIGKDCIGGCSDLVSLQQSGELLTRLK QIGALQ
    • Molecular weight
      13 kDa including tags
    • Amino acids
      1 to 106
    • Tags
      His tag N-Terminus
    • Additional sequence information
      NP_001112362

Specifications

Our Abpromise guarantee covers the use of ab219880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Endotoxin level
    < 1.000 Eu/µg
  • Purity
    >95% by SDS-PAGE .

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.61% Tris, 0.87% Sodium chloride, 0.02% DTT, 5% Trehalose

    Lyophilized from 0.22 µm filtered solution.
    5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names
    • GLRX
    • Glrx1
    • GLRX1_HUMAN
    • Glutaredoxin
    • Glutaredoxin-1
    • Glutaredoxin1
    • Grx
    • Grx 1
    • Grx1
    • MGC117407
    • Thioltransferase
    • Thioltransferase 1
    • Thioltransferase-1
    • Thioltransferase1
    • TTase
    • TTase 1
    • TTase-1
    • TTase1
    see all
  • Function
    Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.
  • Sequence similarities
    Belongs to the glutaredoxin family.
    Contains 1 glutaredoxin domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • SDS-PAGE analysis of ab219880 stained overnight with Coomassie Blue.

    The protein migrates as 12.7 kDa under reducing condition SDS-PAGE.

References

ab219880 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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