Key features and details
- Expression system: Pichia pastoris
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, Functional Studies
Product nameRecombinant human GM-CSF protein
See all GM-CSF proteins and peptides
Biological activityED50 is less than 0.2 ng/ml as determined by the dose-dependent stimulation of the proliferation of Human TF1 cells.
Specific Activity of 5.0 x 106 IU/mg.
Purity> 95 % SDS-PAGE.
Purity is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Endotoxin level< 1.000 Eu/µg
Expression systemPichia pastoris
Protein lengthFull length protein
SequenceAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQ EPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQI ITFESFKENLKDFLLVIPFDCWEPVQE
Predicted molecular weight14 kDa
Amino acids18 to 144
Our Abpromise guarantee covers the use of ab152092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C.
Constituents: 4% Mannitol, 0.16% Tris HCl, 1% Sucrose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionAlways centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 1X PBS. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. For long term storage aliquot and store at < -20°C.
- Colony stimulating factor
- Colony Stimulating Factor 2
- Colony stimulating factor 2 (granulocyte-macrophage)
FunctionCytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Sequence similaritiesBelongs to the GM-CSF family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab152092 has not yet been referenced specifically in any publications.