Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Product nameRecombinant human GM-CSF protein
See all GM-CSF proteins and peptides
Biological activityActivity: The ED50 of ab88382 is typically 0.02 - 0.2 ng/ml as measured in a cell proliferation assay using the human growth factor-dependent TF-1 cell line.
Purity> 95 % SDS-PAGE.
Expression systemHEK 293 cells
Protein lengthFull length protein
SequenceAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMF D LQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPET SC ATQIITFESFKENLKDFLLVIPFDCWEPVQE
Our Abpromise guarantee covers the use of ab88382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 1% Human serum albumin, 10% Trehalose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
- Colony stimulating factor
- Colony Stimulating Factor 2
- Colony stimulating factor 2 (granulocyte-macrophage)
FunctionCytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Sequence similaritiesBelongs to the GM-CSF family.
- Information by UniProt
1D SDS-PAGE of ab88382 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab88382
Lane 2: ab88382 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab88382 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Drop in MWt after treatment with PNGase F indicates the presence of N-linked glycans. Subsequent small drop in MWt after treatment with glycosidase cocktail suggests possible presence of O-linked glycans. Additional bands in lane 2 and lane 3 are glycosidase enzymes.
A sample of ab88382 without carrier protein was reduced and alkylated. 40 μg protein was loaded, focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Spot trains (Deep Purple™ stained) indicates the presence of multiple isoforms of GM-CSF.
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MWt and pI of the protein.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab88382 has not yet been referenced specifically in any publications.