Recombinant Human GNLY/Granulysin protein (ab175462)

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Description

  • Product name

    Recombinant Human GNLY/Granulysin protein

  • Purity

    > 95 % Densitometry.
    ab175462 is purified using Ni-NTA chromatography.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    Escherichia coli

  • Accession

    P22749

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      RLSPEYYDLARAHLRDEEKSCPCLAQEGPQGDLLTKTQELGRDYRTCLTI VQKLKKMVDKPTQRSVSNAATRVCRTGRSRWRDVCRNFMRRYQSRVTQGL VAGETAQQICEDLRLCIPSTGPL

    • Predicted molecular weight

      15 kDa including tags

    • Amino acids

      23 to 145

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab175462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Mass Spectrometry

    ELISA

    SDS-PAGE

  • Mass spectrometry

    LC-MS/MS

  • Form

    Lyophilised

  • Additional notes

     This product was previously labelled as GNLY

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at Room Temperature. Store at +4°C short term (1-2 weeks). Store at -80°C. Avoid freeze / thaw cycle.

    Information available upon request.

  • Reconstitution
    Add 200 µl of deionized water to prepare a working stock solution of 0.5 mg/mL.

General Info

  • Alternative names

    • 519
    • D2S69E
    • GNLY
    • GNLY_HUMAN
    • Granulysin
    • Granulysin precursor
    • LAG 2
    • LAG2
    • Lymphocyte activation gene 2
    • Lymphokine LAG 2
    • Lymphokine LAG-2
    • NKG 5
    • NKG5
    • Protein NKG5
    • T cell activation protein 519
    • T lymphocyte activation gene 519
    • T-cell activation protein 519
    • TLA519
    see all

  • Function

    Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.

  • Tissue specificity

    Expressed in natural killer and T-cells.

  • Sequence similarities

    Contains 1 saposin B-type domain.

  • Post-translational
    modifications

    A 9 kDa form is produced by proteolytic processing of a 15 kDa protein.

  • Cellular localization

    Secreted. Located in the cytotoxic granules of T-cells, which are released upon antigen stimulation.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human GNLY/Granulysin protein (ab175462)
    SDS-PAGE - Recombinant Human GNLY/Granulysin protein (ab175462)

    14% SDS-PAGE analysis of ab175462

    Lane 1: reduced and heated sample (2.5 μg)

    Lane 2: non-reduced and non-heated sample (2.5 μg)

References

ab175462 has not yet been referenced specifically in any publications.

Publishing research using ab175462? Please let us know so that we can cite the reference in this datasheet.

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