Recombinant Human GNLY/Granulysin protein (ab175462)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% Densitometry
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag N-Terminus
- Suitable for: WB, MS, ELISA, SDS-PAGE
Description
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Product name
Recombinant Human GNLY/Granulysin protein -
Purity
> 95 % Densitometry.
ab175462 is purified using Ni-NTA chromatography. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
RLSPEYYDLARAHLRDEEKSCPCLAQEGPQGDLLTKTQELGRDYRTCLTI VQKLKKMVDKPTQRSVSNAATRVCRTGRSRWRDVCRNFMRRYQSRVTQGL VAGETAQQICEDLRLCIPSTGPL -
Predicted molecular weight
15 kDa including tags -
Amino acids
23 to 145 -
Tags
His tag N-Terminus
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Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab175462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Mass Spectrometry
ELISA
SDS-PAGE
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Mass spectrometry
LC-MS/MS -
Form
Lyophilized -
Additional notes
This product was previously labelled as GNLY
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Store at +4°C short term (1-2 weeks). Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.43% Sodium chloride, 99% Phosphate Buffer
ab175462 is 0.4 µm filtered. -
ReconstitutionAdd 200 µl of deionized water to prepare a working stock solution of 0.5 mg/mL.
General Info
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Alternative names
- 519
- D2S69E
- GNLY
see all -
Function
Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis. -
Tissue specificity
Expressed in natural killer and T-cells. -
Sequence similarities
Contains 1 saposin B-type domain. -
Post-translational
modificationsA 9 kDa form is produced by proteolytic processing of a 15 kDa protein. -
Cellular localization
Secreted. Located in the cytotoxic granules of T-cells, which are released upon antigen stimulation. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human GNLY/Granulysin protein (ab175462)
14% SDS-PAGE analysis of ab175462
Lane 1: reduced and heated sample (2.5 μg)
Lane 2: non-reduced and non-heated sample (2.5 μg)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab175462 has not yet been referenced specifically in any publications.
