Recombinant human Granzyme A protein (ab157288)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
      P12544
    • Species
      Human
    • Sequence
      IIGGNEVTPHSRPYMVLLSLDRKTICAGALIAKDWVLTAAHCNLNKRSQV ILGAHSITREEPTKQIMLVKKEFPYPCYDPATREGDLKLLQLMEKAKINK YVTILHLPKKGDDVKPGTMCQVAGWGRTHNSASWSDTLREVNITIIDRKV CNDRNHYNFNPVIGMNMVCAGSLRGGRDSCNGDSGSPLLCEGVFRGVTSF GLENKCGDPRGPGVYILLSKKHLNWIIMTIKGAV
    • Molecular weight
      52 kDa
    • Amino acids
      29 to 262

Associated products

Specifications

Our Abpromise guarantee covers the use of ab157288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific Activity: ~110U/µg protein. One unit is defined as the amount of enzyme that hydrolyzes 1nmol Z-Lys-SBzl per min. at 25°C in 0.05M TRIS, pH 8.0, containing 0.15M NaCl, 0.01% Triton X-100 and 0.3mM DTNB.
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity
    >= 98 % SDS-PAGE.

  • Form
    Liquid
  • Additional notes
    Do not dilute to more than 0.2mg/ml. Do not store dilute solutions (1-1000ng/ml) for more than 24 hours.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 6.00
    Constituents: 0.98% MES, 10% Glycerol, 3.04% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • CTL tryptase
    • CTLA3
    • Cytolytic t cell and natural killer cell specific trypsin like serine protease
    • Cytotoxic T lymphocyte associated serine esterase 3
    • Cytotoxic T lymphocyte proteinase 1
    • Cytotoxic T-lymphocyte proteinase 1
    • Fragmentin 1
    • Fragmentin-1
    • GRAA_HUMAN
    • Granzyme 1 cytotoxic T lymphocyte associated serine esterase 3
    • Granzyme A
    • Granzyme A (Cytotoxic T lymphocyte associated serine esterase 3; Hanukah factor serine protease)
    • Granzyme A (granzyme 1, cytotoxic T lymphocyte associated serine esterase 3)
    • Granzyme A precursor
    • Granzyme-1
    • GZMA
    • H factor
    • Hanukah factor serine protease
    • Hanukkah factor
    • HF
    • HFSP
    • TSP1
    see all
  • Function
    This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Involved in apoptosis.
  • Sequence similarities
    Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization
    Secreted. Cytoplasmic granule.
  • Information by UniProt

References

ab157288 has not yet been referenced specifically in any publications.

Publishing research using ab157288? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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