Recombinant human Granzyme A protein (ab157288)

Submit a review Submit a question

Overview

Description

  • Nature

    Recombinant

  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
      P12544

    • Species

      Human

    • Sequence

      IIGGNEVTPHSRPYMVLLSLDRKTICAGALIAKDWVLTAAHCNLNKRSQV ILGAHSITREEPTKQIMLVKKEFPYPCYDPATREGDLKLLQLMEKAKINK YVTILHLPKKGDDVKPGTMCQVAGWGRTHNSASWSDTLREVNITIIDRKV CNDRNHYNFNPVIGMNMVCAGSLRGGRDSCNGDSGSPLLCEGVFRGVTSF GLENKCGDPRGPGVYILLSKKHLNWIIMTIKGAV

    • Molecular weight

      52 kDa

    • Amino acids

      29 to 262

Specifications

Our Abpromise guarantee covers the use of ab157288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific Activity: ~110U/µg protein. One unit is defined as the amount of enzyme that hydrolyzes 1nmol Z-Lys-SBzl per min. at 25°C in 0.05M TRIS, pH 8.0, containing 0.15M NaCl, 0.01% Triton X-100 and 0.3mM DTNB.
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity

    >= 98 % SDS-PAGE.

  • Form

    Liquid

  • Additional notes

    Do not dilute to more than 0.2mg/ml. Do not store dilute solutions (1-1000ng/ml) for more than 24 hours.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 6.00
    Constituents: 0.98% MES, 10% Glycerol, 3.04% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CTL tryptase
    • CTLA3
    • Cytolytic t cell and natural killer cell specific trypsin like serine protease
    • Cytotoxic T lymphocyte associated serine esterase 3
    • Cytotoxic T lymphocyte proteinase 1
    • Cytotoxic T-lymphocyte proteinase 1
    • Fragmentin 1
    • Fragmentin-1
    • GRAA_HUMAN
    • Granzyme 1 cytotoxic T lymphocyte associated serine esterase 3
    • Granzyme A
    • Granzyme A (Cytotoxic T lymphocyte associated serine esterase 3; Hanukah factor serine protease)
    • Granzyme A (granzyme 1, cytotoxic T lymphocyte associated serine esterase 3)
    • Granzyme A precursor
    • Granzyme-1
    • GZMA
    • H factor
    • Hanukah factor serine protease
    • Hanukkah factor
    • HF
    • HFSP
    • TSP1
    see all

  • Function

    This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Involved in apoptosis.

  • Sequence similarities

    Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.

  • Cellular localization

    Secreted. Cytoplasmic granule.
  • Information by UniProt

References

ab157288 has not yet been referenced specifically in any publications.

Publishing research using ab157288? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab157288.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up