Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
      P62993
    • Species
      Human
    • Molecular weight
      28 kDa
    • Amino acids
      1 to 217

Specifications

Our Abpromise guarantee covers the use of ab116430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF-TOF
  • Purity
    > 95 % SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.40
    Constituent: 99% PBS

General Info

  • Alternative names
    • Abundant SRC homology
    • Adapter protein GRB2
    • ASH
    • Ash protein
    • EGFRBP GRB2
    • Epidermal growth factor receptor binding protein
    • Epidermal growth factor receptor binding protein GRB2
    • GRB 2
    • Grb2
    • GRB2 adapter protein
    • GRB2_HUMAN
    • Grb3 3
    • Growth factor receptor bound protein 2
    • Growth factor receptor bound protein 3
    • Growth factor receptor-bound protein 2
    • HT027
    • MST084
    • MSTP084
    • NCKAP2
    • OTTHUMP00000166096
    • OTTHUMP00000166097
    • OTTHUMP00000166098
    • Protein ash
    • SEM5
    • SH2/SH3 adapter GRB2
    see all
  • Function
    Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
    Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
  • Sequence similarities
    Belongs to the GRB2/sem-5/DRK family.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • Domain
    The SH3 domains mediate interaction with RALGPS1 and SHB.
  • Cellular localization
    Golgi apparatus.
  • Information by UniProt

Images

  • Western blot - GRB2 protein (ab116430)
    Western blot - GRB2 protein (ab116430)
    10% SDS-PAGE stained with Coomassie Blue (Lane 1), immunobloting with anti-6xHis (Lane 2)

References

ab116430 has not yet been referenced specifically in any publications.

Publishing research using ab116430? Please let us know so that we can cite the reference in this datasheet.

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