Recombinant human Gremlin 1 protein (ab139770)
Key features and details
- Expression system: CHO cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, HPLC, SDS-PAGE
Description
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Product name
Recombinant human Gremlin 1 protein
See all Gremlin 1 proteins and peptides -
Biological activity
Determined by its ability to inhibit BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 for this effect is 0.07-0.11 µg/ml.
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Purity
> 90 % SDS-PAGE.
The purity of ab139770 is greater than 90% by SDS-PAGE gel and HPLC analyses. -
Expression system
CHO cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
KKKGSQGAIP PPDKAQHNDS EQTQSPQQPG SRNRGRGQGR GTAMPGEEVL ESSQEALHVT ERKYLKRDWC KTQPLKQTIH EEGCNSRTII NRFCYGQCNS FYIPRHIRKE EGSFQSCSFC KPKKFTTMMV TLNCPELQPP TKKKRVTRVK QCRCISIDLD -
Predicted molecular weight
18 kDa -
Amino acids
25 to 184
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab139770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
HPLC
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 0.16% Sodium phosphate, 0.29% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionCentrifuge vial prior to opening. Reconstitute in water to 0.1-1.0 mg/ml and store at 2°C to 8°C for up to 1 week. Do not vortex. Extended storage: Follow reconstitution with further dilution in a buffer containing a carrier protein (example 0.1% BSA) and store at -20°C to -80°C for up to 3 months.
General Info
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Alternative names
- BMP antagonist 1
- Cell proliferation inducing gene 2 protein
- Cell proliferation-inducing gene 2 protein
see all -
Function
Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis. -
Tissue specificity
Highly expressed in small intestine, fetal brain and colon. Expression is restricted to intestinal subepithelial myofibroblasts (ISEMFs) at the crypt base. In subjects with HMPS1, by contrast, GREM1 is expressed, not only in basal ISEMFs, but also at very high levels in epithelial cells (predominantly colonocytes), with expression extending most of the way up the sides of the crypt. Weakly expressed in brain, ovary, prostate, pancreas and skeletal muscle. In brain found in the region localized around the internal capsule in the large subcortical nuclei, including caudate, putamen, substantia nigra, thalamus and subthalamus. Predominantly expressed in normal cells including neurons, astrocytes and fibroblasts. -
Involvement in disease
Polyposis syndrome, mixed hereditary 1 -
Sequence similarities
Belongs to the DAN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab139770 has not yet been referenced specifically in any publications.