Overview

Description

  • Nature

    Recombinant
  • Source

    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Molecular weight

      93 kDa including tags
    • Amino acids

      1 to 589

Specifications

Our Abpromise guarantee covers the use of ab125576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The specific activity of ab125576 was determined to be 11 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity

    > 80 % Densitometry.
    Purity determined to be >80% by densitometry.
  • Form

    Liquid
  • Additional notes

    ab204876 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • FLJ32135
    • G protein coupled receptor kinase 6
    • G protein coupled receptor kinase GRK6
    • G protein-coupled receptor kinase 6
    • G protein-coupled receptor kinase GRK6
    • Gprk6
    • Grk6
    • GRK6_HUMAN
    see all
  • Function

    Specifically phosphorylates the activated forms of G protein-coupled receptors.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • Post-translational
    modifications

    It is uncertain whether palmitoylation is on Cys-561 and/or Cys-562 and/or Cys-565.
  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • SDS-PAGE analysis of ab125576.
  • The specific activity of ab125576 was determined to be 11 nmol/min/mg by Kinase Assay.

References

ab125576 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab125576.
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