Bioactive grade

Recombinant human Growth Hormone protein (Active) (ab51232)

Description

  • Product name

    Recombinant human Growth Hormone protein (Active)
    See all Growth Hormone proteins and peptides
  • Biological activity

    Measured in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 for this effect is less or equal to 0.2 ng/ml.

  • Purity

    > 95 % SDS-PAGE.
    Recombinant human Growth Hormone was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQT SLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANS LVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNS HNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF
    • Predicted molecular weight

      22 kDa
    • Amino acids

      27 to 217
    • Additional sequence information

      Full length mature chain without signal peptide. NP_000506.

Specifications

Our Abpromise guarantee covers the use of ab51232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.40
    Constituents: 0.0134% PBS, 1.6016% Mannitol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • gH
    • GH-N
    • GH1
    • GHB5
    • GHN
    • Growth hormone
    • Growth hormone 1
    • Growth hormone B5
    • Growth hormone, normal
    • Growth hormone, pituitary
    • HG1
    • hGH-N
    • IGHD1B
    • Pituitary growth hormone
    • RNGHGP
    • SOMA_HUMAN
    • Somatotropin
    see all
  • Function

    Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • Involvement in disease

    Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
    Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy.
  • Sequence similarities

    Belongs to the somatotropin/prolactin family.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • 15% SDS-PAGE - Recombinant human Growth Hormone protein (Active) (ab51232, 3 µg).

    Reducing conditions. Visualized by coomassie blue stain.

  • All lanes : Anti-Growth Hormone antibody [GH-1] (ab9821) at 1/500 dilution

    Lane 1 : Recombinant human Growth Hormone protein (Active) (ab51232) at 0.1 µg
    Lane 2 : Recombinant human Growth Hormone protein (Active) (ab51232) at 0.01 µg

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 2 minutes

References

ab51232 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

ab51232 has not been used for subcutaneous administration in vivo experiments, but pharmacokinetics test has been performed in mouse without any problems. Thus, there shouldn’t be any problems using it for in vivo subcutaneous administration experiments with rats but we cannot guarantee it.

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