Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, HPLC
Product nameRecombinant human Growth Hormone protein (Animal Free)
See all Growth Hormone proteins and peptides
Determined by its ability to stimulate the proliferation of rat Nb2-11 cells. The expected ED50 is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
Purity> 98 % SDS-PAGE.
>98% by HPLC.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceFPTIPLSRLF DNAMLRAHRL HQLAFDTYQE FEEAYIPKEQ KYSFLQNPQT SLCFSESIPT PSNREETQQK SNLELLRISL LLIQSWLEPV QFLRSVFANS LVYGASDSNV YDLLKDLEEG IQTLMGRLED GSPRTGQIFK QTYSKFDTNS HNDDALLKNY GLLYCFRKDM DKVETFLRIV QCRSVEGSCG F
Predicted molecular weight22 kDa
Amino acids27 to 217
Additional sequence informationThis product is for the mature full length protein. The signal peptide is not included.
Our Abpromise guarantee covers the use of ab217403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionCentrifuge vial prior to opening. Reconstitute in water to 0.1-1.0 mg/ml. Do not vortex. Note: Allow the reconstituted vial to sit at room temperature for 1 hour before use. Do not vortex. Store at 2°C to 8°C for 1 week, or prepare for extended storage. For Extended Storage: Follow reconstitution with further dilution in a buffer containing a carrier protein (example PBS containing 5% Trehalose). Store working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
FunctionPlays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
Involvement in diseaseDefects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy.
Sequence similaritiesBelongs to the somatotropin/prolactin family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab217403 has not yet been referenced specifically in any publications.