Description

  • Product name

    Recombinant human GRX2 protein
    See all GRX2 proteins and peptides
  • Biological activity

    Specific activity : 45 U/mg One unit will cause the oxidation of 1µmole of NADPH per min.
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Amino acids

      41 to 164

Specifications

Our Abpromise guarantee covers the use of ab82665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

     This product was previously labelled as Glutaredoxin 2

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.00
    Constituents: 0.476% HEPES, 10% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • bA101E13.1
    • bA101E13.1 (GRX2 glutaredoxin (thioltransferase) 2)
    • CGI133
    • Glrx2
    • GLRX2_HUMAN
    • Glutaredoxin-2
    • Glutaredoxin-2, mitochondrial
    • GRX2
    • mitochondrial
    see all
  • Function

    Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.
  • Tissue specificity

    Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta and lung. Not expressed in peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the glutaredoxin family.
    Contains 1 glutaredoxin domain.
  • Cellular localization

    Mitochondrion and Nucleus.
  • Information by UniProt

Images

  • ab82665 at 1µg on SDS-PAGE.

References

ab82665 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Here is how the lab performs the activity assay:


100 mM Tris-Cl(pH8.0), 2 mM EDTA, 0.1 mg/ml BSA, 1 mM GSH, 0.2 mM NADPH, 6 ug/ml yeast GR, 0.7 mM HED and X ug Grx2 protein.


Here are some other publications describing measuring glutaredoxin activity (at 340nm):

http://lqma.ifas.ufl.edu/Publication/Sundaram-09.pdf

http://www.jbc.org/content/283/18/11913.full

http://www.jbc.org/content/257/12/6686.full.pdf+html

http://www.fasebj.org/content/early/2003/04/02/fj.02-0771fje.full.pdf

http://www.biochemj.org/bj/429/0085/4290085.pdf

http://www.jbc.org/content/early/2000/04/10/jbc.M909235199.full.pdf

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