Recombinant human GSK3 beta protein (Active) (ab60863)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Affinity purified
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies, WB
Description
-
Product name
Recombinant human GSK3 beta protein (Active)
See all GSK3 beta proteins and peptides -
Biological activity
148 nmol/min/mg
-
Purity
> 70 % Affinity purified.
Purified by affinity chromatography -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MSGRPRTTSF AESCKPVQQP SAFGSMKVSR DKDGSKVTTV VATPGQGPDR PQEVSYTDTK VIGNGSFGVV YQAKLCDSGE LVAIKKVLQD KRFKNRELQI MRKLDHCNIV RLRYFFYSSG EKKDEVYLNL VLDYVPETVY RVARHYSRAK QTLPVIYVKL YMYQLFRSLA YIHSFGICHR DIKPQNLLLD PDTAVLKLCD FGSAKQLVRG EPNVSYICSR YYRAPELIFG ATDYTSSIDV WSAGCVLAEL LLGQPIFPGD SGVDQLVEII KVLGTPTREQ IREMNPNYTE FKFPQIKAHP WTKDSSGTGH FTSGVRVFRP RTPPEAIALC SRLLEYTPTA RLTPLEACAH SFFDELRDPN VKLPNGRDTP ALFNFTTQEL SSNPPLATIL IPPHARIQAA ASTPTNATAA SDANTGDRGQ TNNAASASAS NST -
Predicted molecular weight
73 kDa including tags -
Amino acids
1 to 420 -
Tags
GST tag N-Terminus -
Additional sequence information
(Genbank Number NM_002093)
-
Associated products
-
Related Products
-
Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab60863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
Functional Studies
Western blot
-
Form
Liquid -
Additional notes
ab204860 (Glycogen synthase 1 peptide) can be utilized as a substrate for assessing kinase activity.
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.307% GlutathioneThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- Glycogen Synthase Kinase 3 Beta
- Glycogen synthase kinase-3 beta
- GSK 3 beta
see all -
Function
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. -
Tissue specificity
Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216. -
Cellular localization
Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane. - Information by UniProt
Images
-
Functional Studies - Recombinant human GSK3 beta protein (Active) (ab60863)The specific activity of GSK3 beta (ab60863) was determined to be 218 nmol/min/mg as per activity assay protocol and was equivalent to 210 nmol/min/mg as per radiometric assay
-
SDS-PAGE - Recombinant human GSK3 beta protein (Active) (ab60863)SDS PAGE analysis of ab60863 -
SDS-PAGE - Recombinant human GSK3 beta protein (Active) (ab60863)SDS PAGE analysis of ab60863 -
Functional Studies - Recombinant human GSK3 beta protein (Active) (ab60863)Activity of ab60863: 148 nmol/min/mg (as per activity assay protocol).
-
SDS-PAGE - Recombinant human GSK3 beta protein (Active) (ab60863)The purity was determined to be >75% by densiometry.
Approximate MW: 73 kDa.
-
Western blot - Recombinant human GSK3 beta protein (Active) (ab60863)ab60863 is a tagged recombinant protein with an expected molecular weight of 73kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (5)
ab60863 has been referenced in 5 publications.
- Guo S et al. TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress. Cell Death Dis 12:42 (2021). PubMed: 33414446
- Nowak DG et al. The PHLPP2 phosphatase is a druggable driver of prostate cancer progression. J Cell Biol 218:1943-1957 (2019). PubMed: 31092557
- Han H et al. Small-Molecule MYC Inhibitors Suppress Tumor Growth and Enhance Immunotherapy. Cancer Cell 36:483-497.e15 (2019). PubMed: 31679823
- Li L et al. A TFEB nuclear export signal integrates amino acid supply and glucose availability. Nat Commun 9:2685 (2018). PubMed: 29992949
- Suryo Rahmanto A et al. FBW7 suppression leads to SOX9 stabilization and increased malignancy in medulloblastoma. EMBO J 35:2192-2212 (2016). PubMed: 27625374