Recombinant human GSK3 beta protein (Active) (ab60863)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameRecombinant human GSK3 beta protein (Active)
See all GSK3 beta proteins and peptides -
Protein lengthFull length protein
Description
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NatureRecombinant
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SourceBaculovirus infected Sf9 cells
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Amino Acid Sequence
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Accession
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SpeciesHuman
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Molecular weight73 kDa
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TagsGST tag N-Terminus
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Associated products
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Related Products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab60863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Biological activity
Specific Activity: 187 nmol/min/mg
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Applications
SDS-PAGE
Functional Studies
Western blot
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FormLiquid
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Additional notes
ab204860 (Glycogen synthase 1 peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: None
Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Glycogen Synthase Kinase 3 Beta
- Glycogen synthase kinase-3 beta
- GSK 3 beta
see all -
FunctionParticipates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.
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Tissue specificityExpressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.
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Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216. -
Cellular localizationCytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
- Information by UniProt
Images
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Functional Studies - Recombinant human GSK3 beta protein (Active) (ab60863)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human GSK3 beta protein (Active) (ab60863)ab60863 on SDS-PAGE, MW ~73 kDa. -
Western blot - Recombinant human GSK3 beta protein (Active) (ab60863)ab60863 is a tagged recombinant protein with an expected molecular weight of 73kDa.
References
This product has been referenced in:
- Suryo Rahmanto A et al. FBW7 suppression leads to SOX9 stabilization and increased malignancy in medulloblastoma. EMBO J 35:2192-2212 (2016). Read more (PubMed: 27625374) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"