Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, MS, SDS-PAGE
Product nameRecombinant human GSTM5 protein
See all GSTM5 proteins and peptides
Specific activity is > 90,000 pmol/min/µg, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25 degC.
Purity> 95 % SDS-PAGE.
ab207801 was expressed in E.coli and purified by using conventional chromatography techniques
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMGSSHHHHHHSSGLVPRGSHMGSHMPMTLGYWDIRGLAHAIRLLLEYTDS SYVEKKYTLGDAPDYDRSQWLNEKFKLGLDFPNLPYLIDGAHKITQSNAI LRYIARKHNLCGETEEEKIRVDILENQVMDNHMELVRLCYDPDFEKLKPK YLEELPEKLKLYSEFLGKRPWFAGDKITFVDFLAYDVLDMKRIFEPKCLD AFLNLKDFISRFEGLKKISAYMKSSQFLRGLLFGKSATWNSK
Predicted molecular weight28 kDa including tags
Amino acids1 to 218
TagsHis tag N-Terminus
Additional sequence informationNP_000842.
Our Abpromise guarantee covers the use of ab206801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.32% Tris HCl, 0.02% DTT, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Glutathione S alkyltransferase M5
- Glutathione S aralkyltransferase M5
- Glutathione S aryltransferase M5
FunctionConjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Sequence similaritiesBelongs to the GST superfamily. Mu family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab206801 has not yet been referenced specifically in any publications.