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As you know for transient transfection you may deliver plasmids to cells or directly transfect cells with recombinant proteins. I would use thesecondmethod. I think the H-Ras protein you introduced is well but how about K-Ras and N-Ras? Bests
Asked on Apr 23 2012
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The ab90765 and ab90768 are also active proteins and can be used in functional assays.
ab96817 is not an active protein.
Please note that we haven't tried these proteins in functional assay so the optimal conditions have to be determined by the end user.
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Answered on Apr 24 2012