Recombinant Human HADHB protein (His tag) (denatured) (ab202260)

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SDS-PAGE - Recombinant Human HADHB protein (His tag) (denatured) (ab202260)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human HADHB protein (His tag) (denatured)
      See all HADHB proteins and peptides

    • Purity

      > 90 % SDS-PAGE.

    • Expression system

      Escherichia coli

    • Accession

      P55084

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGSAAPAVQTKTKKTLAKPNIRNVVVVDGVRTPFLLSGTSYKDLMPHDLA RAALTGLLHRTSVPKEVVDYIIFGTVIQEVKTSNVAREAALGAGFSDKTP AHTVTMACISANQAMTTGVGLIASGQCDVIVAGGVELMSDVPIRHSRKMR KLMLDLNKAKSMGQRLSLISKFRFNFLAPELPAVSEFSTSETMGHSADRL AAAFAVSRLEQDEYALRSHSLAKKAQDEGLLSDVVPFKVPGKDTVTKDNG IRPSSLEQMAKLKPAFIKPYGTVTAANSSFLTDGASAMLIMAEEKALAMG YKPKAYLRDFMYVSQDPKDQLLLGPTYATPKVLEKAGLTMNDIDAFEFHE AFSGQILANFKAMDSDWFAENYMGRKTKVGLPPLEKFNNWGGSLSLGHPF GATGCRLVMAAANRLRKEGGQYGLVAACAAGGQGHAMIVEAYPK

      • Predicted molecular weight

        50 kDa including tags

      • Amino acids

        34 to 474

      • Tags

        His tag N-Terminus

      • Additional sequence information

        Mature protein without signal peptide. NCBI Accession No.: NP_000174.

    • Description

      Recombinant Human HADHB protein (His tag)

    Specifications

    Our Abpromise guarantee covers the use of ab202260 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 90% Phosphate Buffer, 10% Glycerol (glycerin, glycerine)

    General Info

    • Alternative names

      • 2 enoyl Coenzyme A (CoA) hydratase beta subunit
      • 3 ketoacyl Coenzyme A (CoA) thiolase of mitochondrial trifunctional protein beta subunit
      • 3 ketoacyl Coenzyme A thiolase
      • 3 ketoacyl Coenzyme A thiolase of mitochondrial trifunctional protein beta subunit
      • 3-ketoacyl-CoA thiolase
      • Acetyl CoA acyltransferase
      • Acetyl-CoA acyltransferase
      • Beta ketothiolase
      • Beta-ketothiolase
      • ECHB
      • ECHB_HUMAN
      • HADH
      • Hadhb
      • Hydroxyacyl CoA dehydrogenase/3 ketoacyl CoA thiolase/enoyl CoA hydratase (trifunctional protein), beta subunit
      • Hydroxyacyl Coenzyme A (CoA) dehydrogenase beta subunit
      • Hydroxyacyl Coenzyme A dehydrogenase
      • Hydroxyacyl Coenzyme A dehydrogenase beta subunit
      • Hydroxyacyl Coenzyme A dehydrogenase/3 ketoacyl Coenzyme A thiolase/enoyl Coenzyme A hydratase (trifunctional protein) beta subunit
      • MGC87480
      • Mitochondrial trifunctional enzyme beta subunit
      • Mitochondrial trifunctional protein beta subunit
      • MSTP 029
      • MSTP029
      • MTPB
      • TP beta
      • TP-beta
      • TPbeta
      • Trifunctional enzyme subunit beta
      • Trifunctional enzyme subunit beta mitochondrial
      • Trifunctional protein
      see all

    • Pathway

      Lipid metabolism; fatty acid beta-oxidation.

    • Involvement in disease

      Defects in HADHB are a cause of trifunctional protein deficiency (TFP deficiency) [MIM:609015]. The clinical manifestations are very variable and include hypoglycemia, cardiomyopathy and sudden death. Phenotypes with mainly hepatic and neuromyopathic involvement can also be distinguished. Biochemically, TFP deficiency is defined by the loss of all three enzyme activities of the TFP complex.

    • Sequence similarities

      Belongs to the thiolase family.

    • Cellular localization

      Mitochondrion.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human HADHB protein (His tag) (denatured) (ab202260)
      SDS-PAGE - Recombinant Human HADHB protein (His tag) (denatured) (ab202260)

      15% SDS-PAGE analysis of ab202260 (3 µg)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab202260? Please let us know so that we can cite the reference in this datasheet.

    ab202260 has not yet been referenced specifically in any publications.

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