Recombinant human HAO1/GOX protein (Active) (ab241318)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, MS
Description
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Product name
Recombinant human HAO1/GOX protein (Active)
See all HAO1/GOX proteins and peptides -
Biological activity
Specific activity is > 3,000 pmol/min/µg, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25°C.
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Purity
> 95 % SDS-PAGE.
Purified using conventional chromatography. -
Endotoxin level
< 0.100 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMLPRLICINDYEQH AKSVLPKSIYDYYRSGANDEETLADNIAAFSRWKLYPRMLRNVAETDLST SVLGQRVSMPICVGATAMQRMAHVDGELATVRACQSLGTGMMLSSWATSS IEEVAEAGPEALRWLQLYIYKDREVTKKLVRQAEKMGYKAIFVTVDTPYL GNRLDDVRNRFKLPPQLRMKNFETSTLSFSPEENFGDDSGLAAYVAKAID PSISWEDIKWLRRLTSLPIVAKGILRGDDAREAVKHGLNGILVSNHGARQ LDGVPATIDVLPEIVEAVEGKVEVFLDGGVRKGTDVLKALALGAKAVFVG RPIVWGLAFQGEKGVQDVLEILKEEFRLAMALSGCQNVKVIDKTLVRKNP LAVSKI -
Predicted molecular weight
45 kDa including tags -
Amino acids
1 to 370 -
Tags
His tag N-Terminus -
Additional sequence information
NP_060015.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab241318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.32% Tris HCl, 20% Glycerol (glycerin, glycerine), 2.9% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- (S) 2 hydroxy acid oxidase
- Glycolate oxidase
- GOX
see all -
Function
Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate. -
Tissue specificity
Liver. -
Pathway
Organic acid metabolism; glycolate degradation; 3-phospho-D-glycerate from glycolate: step 1/4. -
Sequence similarities
Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.
Contains 1 FMN hydroxy acid dehydrogenase domain. -
Cellular localization
Peroxisome. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab241318 has not yet been referenced specifically in any publications.