Recombinant Human HAO1/GOX protein (His tag) (ab219876)

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Description

  • Product name

    Recombinant Human HAO1/GOX protein (His tag)
    See all HAO1/GOX proteins and peptides

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    Q9UJM8

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      LPRLICINDYEQHAKSVLPKSIYDYYRSGANDEETLADNIAAFSRWKLYP RMLRNVAETDLSTSVLGQRVSMPICVGATAMQRMAHVDGELATVRACQSL GTGMMLSSWATSSIEEVAEAGPEALRWLQLYIYKDREVTKKLVRQAEKMG YKAIFVTVDTPYLGNRLDDVRNRFKLPPQLRMKNFETSTLSFSPEENFGD DSGLAAYVAKAIDPSISWEDIKWLRRLTSLPIVAKGILRGDDAREAVKHG LNGILVSNHGARQLDGVPATIDVLPEIVEAVEGKVEVFLDGGVRKGTDVL KALALGAKAVFVGRPIVWGLAFQGEKGVQDVLEILKEEFRLAMALSGCQN VKVIDKTLVRKNPLAVSKI

    • Predicted molecular weight

      42 kDa including tags

    • Amino acids

      2 to 370

    • Tags

      His tag N-Terminus

    • Additional sequence information

      AAI13666.

Specifications

Our Abpromise guarantee covers the use of ab219876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised

  • Additional notes

    Protein previously labeled as HAO1.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.61% Tris, 0.87% Sodium chloride, 5% Trehalose

    Lyophilized from 0.22 µm filtered solution.
    5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • (S) 2 hydroxy acid oxidase
    • Glycolate oxidase
    • GOX
    • GOX1
    • HAO1
    • HAOX1
    • HAOX1_HUMAN
    • Hydroxyacid oxidase 1
    • MGC142225
    • MGC142227
    • OTTHUMP00000030231
    see all

  • Function

    Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.

  • Tissue specificity

    Liver.

  • Pathway

    Organic acid metabolism; glycolate degradation; 3-phospho-D-glycerate from glycolate: step 1/4.

  • Sequence similarities

    Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.
    Contains 1 FMN hydroxy acid dehydrogenase domain.

  • Cellular localization

    Peroxisome.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human HAO1/GOX protein (His tag) (ab219876)
    SDS-PAGE - Recombinant Human HAO1/GOX protein (His tag) (ab219876)

    SDS-PAGE analysis of ab219876 stained overnight with Coomassie Blue.

    The protein migrates as 41.7 kDa under reducing condition SDS-PAGE.

References

ab219876 has not yet been referenced specifically in any publications.

Publishing research using ab219876? Please let us know so that we can cite the reference in this datasheet.

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