Recombinant Human HAO1/GOX protein (His tag) (ab219876)

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SDS-PAGE - Recombinant Human HAO1/GOX protein (His tag) (ab219876)

    Key features and details

    • Expression system: HEK 293 cells
    • Purity: > 95% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human HAO1/GOX protein (His tag)
      See all HAO1/GOX proteins and peptides

    • Purity

      > 95 % SDS-PAGE.

    • Endotoxin level

      < 1.000 Eu/µg

    • Expression system

      HEK 293 cells

    • Accession

      Q9UJM8

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        LPRLICINDYEQHAKSVLPKSIYDYYRSGANDEETLADNIAAFSRWKLYP RMLRNVAETDLSTSVLGQRVSMPICVGATAMQRMAHVDGELATVRACQSL GTGMMLSSWATSSIEEVAEAGPEALRWLQLYIYKDREVTKKLVRQAEKMG YKAIFVTVDTPYLGNRLDDVRNRFKLPPQLRMKNFETSTLSFSPEENFGD DSGLAAYVAKAIDPSISWEDIKWLRRLTSLPIVAKGILRGDDAREAVKHG LNGILVSNHGARQLDGVPATIDVLPEIVEAVEGKVEVFLDGGVRKGTDVL KALALGAKAVFVGRPIVWGLAFQGEKGVQDVLEILKEEFRLAMALSGCQN VKVIDKTLVRKNPLAVSKI

      • Predicted molecular weight

        42 kDa including tags

      • Amino acids

        2 to 370

      • Tags

        His tag N-Terminus

      • Additional sequence information

        AAI13666.

    Specifications

    Our Abpromise guarantee covers the use of ab219876 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized

    • Additional notes

      Protein previously labeled as HAO1.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.

      pH: 7.4
      Constituents: 0.61% Tris, 0.87% Sodium chloride, 5% Trehalose

      Lyophilized from 0.22 µm filtered solution.
      5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%

    • Reconstitution
      Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

    General Info

    • Alternative names

      • (S) 2 hydroxy acid oxidase
      • Glycolate oxidase
      • GOX
      • GOX1
      • HAO1
      • HAOX1
      • HAOX1_HUMAN
      • Hydroxyacid oxidase 1
      • MGC142225
      • MGC142227
      • OTTHUMP00000030231
      see all

    • Function

      Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.

    • Tissue specificity

      Liver.

    • Pathway

      Organic acid metabolism; glycolate degradation; 3-phospho-D-glycerate from glycolate: step 1/4.

    • Sequence similarities

      Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.
      Contains 1 FMN hydroxy acid dehydrogenase domain.

    • Cellular localization

      Peroxisome.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human HAO1/GOX protein (His tag) (ab219876)
      SDS-PAGE - Recombinant Human HAO1/GOX protein (His tag) (ab219876)

      SDS-PAGE analysis of ab219876 stained overnight with Coomassie Blue.

      The protein migrates as 41.7 kDa under reducing condition SDS-PAGE.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab219876? Please let us know so that we can cite the reference in this datasheet.

    ab219876 has not yet been referenced specifically in any publications.

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