Key features and details
- Expression system: Escherichia coli
- Purity: >= 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human HBEGF/DTR protein (Active)
See all HBEGF/DTR proteins and peptides
The ED50 was determined by cell proliferation assay (using balb/c 3T3) and was found to be ≤1.0ng/ml, corresponding to a specific activity of ≥ 1×106 units/mg.
Purity>= 95 % SDS-PAGE.
Endotoxin level< 1.000 Eu/µg
Expression systemEscherichia coli
Protein lengthFull length protein
Molecular weight informationSDS-PAGE molecular weight: ~10.1kDa
Additional sequence informationMature chain.
Our Abpromise guarantee covers the use of ab268609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
It is recommended to reconstitute in sterile PBS to 250 µg/ml.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Lyophilized from a 0.2 micron solution.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionA quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
- Diphtheria toxin receptor
- diphtheria toxin receptor (heparin-binding EGF-like growth factor)
- diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)
FunctionMay be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
Sequence similaritiesContains 1 EGF-like domain.
modificationsSeveral N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).
Cellular localizationCell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab268609 has not yet been referenced specifically in any publications.