Recombinant human HC-II protein (ab191977)
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Description
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Product name
Recombinant human HC-II protein
See all HC-II proteins and peptides -
Biological activity
Measured by its ability to inhibit Recombinant Human Coagulation Factor II/Thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC. The IC50 value is <1.8 nM.
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
GSKGPLDQLEKGGETAQSADPQWEQLNNKNLSMPLLPADFHKENTVTNDW IPEGEEDDDYLDLEKIFSEDDDYIDIVDSLSVSPTDSDVSAGNILQLFHG KSRIQRLNILNAKFAFNLYRVLKDQVNTFDNIFIAPVGISTAMGMISLGL KGETHEQVHSILHFKDFVNASSKYEITTIHNLFRKLTHRLFRRNFGYTLR SVNDLYIQKQFPILLDFKTKVREYYFAEAQIADFSDPAFISKTNNHIMKL TKGLIKDALENIDPATQMMILNCIYFKGSWVNKFPVEMTHNHNFRLNERE VVKVSMMQTKGNFLAANDQELDCDILQLEYVGGISMLIVVPHKMSGMKTL EAQLTPRVVERWQKSMTNRTREVLLPKFKLEKNYNLVESLKLMGIRMLFD KNGNMAGISDQRIAIDLFKHQGTITVNEEGTQATTVTTVGFMPLSTQVRF TVDRPFLFLIYEHRTSCLLFMGRVANPSRS -
Predicted molecular weight
56 kDa including tags -
Amino acids
20 to 499 -
Tags
His tag C-Terminus -
Additional sequence information
Mature protein with out the signal peptide. (Accession # AAH35028)
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab191977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilised -
Additional notes
Protein previously labeled as Heparin Cofactor II.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C long term. Please see notes section.
pH: 7.50
Constituents: 0.61% Tris, 0.87% Sodium chloride
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile deionized water to a stock solution of 100ug/ml. After reconstitution store under sterile conditions at -70 degrees.
General Info
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Alternative names
- D22S673
- HC II
- HC-II
see all -
Function
Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.
Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils. -
Tissue specificity
Expressed predominantly in liver. Also present in plasma. -
Involvement in disease
Thrombophilia due to heparin cofactor 2 deficiency -
Sequence similarities
Belongs to the serpin family. -
Domain
The N-terminal acidic repeat region mediates, in part, the glycosaminoglycan-accelerated thrombin inhibition. -
Post-translational
modificationsPhosphorylation sites are present in the extracellular medium. - Information by UniProt
Images
Datasheets and documents
References
ab191977 has not yet been referenced specifically in any publications.