Bioactive grade

Recombinant human HC-II protein (ab191977)

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Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Active: Yes
  • Tags: His tag C-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human HC-II protein
    See all HC-II proteins and peptides

  • Biological activity

    Measured by its ability to inhibit Recombinant Human Coagulation Factor II/Thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC. The IC50 value is <1.8 nM.

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    P05546

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      GSKGPLDQLEKGGETAQSADPQWEQLNNKNLSMPLLPADFHKENTVTNDW IPEGEEDDDYLDLEKIFSEDDDYIDIVDSLSVSPTDSDVSAGNILQLFHG KSRIQRLNILNAKFAFNLYRVLKDQVNTFDNIFIAPVGISTAMGMISLGL KGETHEQVHSILHFKDFVNASSKYEITTIHNLFRKLTHRLFRRNFGYTLR SVNDLYIQKQFPILLDFKTKVREYYFAEAQIADFSDPAFISKTNNHIMKL TKGLIKDALENIDPATQMMILNCIYFKGSWVNKFPVEMTHNHNFRLNERE VVKVSMMQTKGNFLAANDQELDCDILQLEYVGGISMLIVVPHKMSGMKTL EAQLTPRVVERWQKSMTNRTREVLLPKFKLEKNYNLVESLKLMGIRMLFD KNGNMAGISDQRIAIDLFKHQGTITVNEEGTQATTVTTVGFMPLSTQVRF TVDRPFLFLIYEHRTSCLLFMGRVANPSRS

    • Predicted molecular weight

      56 kDa including tags

    • Amino acids

      20 to 499

    • Tags

      His tag C-Terminus

    • Additional sequence information

      Mature protein with out the signal peptide. (Accession # AAH35028)

Specifications

Our Abpromise guarantee covers the use of ab191977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilized

  • Additional notes

    Protein previously labeled as Heparin Cofactor II.

    Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C long term. Please see notes section.

    pH: 7.4
    Constituents: 0.61% Tris, 0.87% Sodium chloride

    5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

General Info

  • Alternative names

    • D22S673
    • HC II
    • HC-II
    • HC2
    • HCF2
    • HEP2_HUMAN
    • Heparin cofactor 2
    • Heparin cofactor II
    • HLS2
    • Leuserpin 2
    • LS2
    • Protease inhibitor leuserpin 2
    • Protease inhibitor leuserpin-2
    • serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1
    • Serpin D1
    • serpin peptidase inhibitor, clade D (heparin cofactor), member 1
    • SERPIND1
    • THPH10
    see all

  • Function

    Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.
    Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils.

  • Tissue specificity

    Expressed predominantly in liver. Also present in plasma.

  • Involvement in disease

    Thrombophilia due to heparin cofactor 2 deficiency

  • Sequence similarities

    Belongs to the serpin family.

  • Domain

    The N-terminal acidic repeat region mediates, in part, the glycosaminoglycan-accelerated thrombin inhibition.

  • Post-translational
    modifications

    Phosphorylation sites are present in the extracellular medium.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human HC-II protein (ab191977)
    SDS-PAGE - Recombinant human HC-II protein (ab191977)

    SDS-PAGE analysis of reduced ab191977 and staining overnight with Coomassie Blue.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab191977? Please let us know so that we can cite the reference in this datasheet.

ab191977 has not yet been referenced specifically in any publications.

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