Key features and details
- Expression system: Baculovirus
- Purity: > 50% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, DDDDK tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human HDAC1 protein
See all HDAC1 proteins and peptides
Specific Activity: 460 pmol/min/µg.
Purity> 50 % SDS-PAGE.
Purity is >50% as determined by SDS-PAGE.
Protein lengthFull length protein
Predicted molecular weight56 kDa
Amino acids1 to 482
TagsHis tag C-Terminus , DDDDK tag C-Terminus
Our Abpromise guarantee covers the use of ab104121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.395% Tris HCl, 0.05% Tween, 10% Glycerol, 0.754% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- GON 10
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
Tissue specificityUbiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
modificationsSumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome.
- Information by UniProt
10% SDS-PAGE showing ab104121 at approximately 56kDa (1µg).
HDAC1 specific activity. HDAC reaction was carried out for 30 minutes at 37°C in a solution containing 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA, 20 µM Fluorogenic HDAC substrate, and varying amounts of HDAC1 from 0-80 ng. This was followed by treatment with 50 µL of HDAC developer for 15 minutes at room temperature. Fluorescence intensity is measured at ex360/em460.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab104121 has not yet been referenced specifically in any publications.