Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus
  • Amino Acid Sequence
    • Accession
      Q92769
    • Species
      Human
    • Molecular weight
      92 kDa including tags
    • Amino acids
      1 to 488

Specifications

Our Abpromise guarantee covers the use of ab101662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

  • Purity
    > 70 % SDS-PAGE.
    The purity of ab101662 was determined to be >70% by densitometry.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

General Info

  • Alternative names
    • D10Wsu179e
    • HD 2
    • HD2
    • HDAC 2
    • Hdac2
    • HDAC2_HUMAN
    • Histone deacetylase 2
    • Histone deacetylase 2 (HD2)
    • OTTHUMP00000017046
    • OTTHUMP00000227077
    • OTTHUMP00000227078
    • RPD3
    • transcriptional regulator homolog RPD3
    • YAF1
    • YY1 associated factor 1
    • YY1 transcription factor binding protein
    • Yy1bp
    see all
  • Function
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
    Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
  • Tissue specificity
    Widely expressed; lower levels in brain and lung.
  • Sequence similarities
    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications
    S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
  • Cellular localization
    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE - HDAC2 protein (Active) (ab101662)
    SDS-PAGE - HDAC2 protein (Active) (ab101662)
    The purity of ab101662 was determined to be >70% as determined by densitometry.
    Approximate molecular weight is ~92 kDa (with tag).
  • Functional Studies - HDAC2 protein (Active) (ab101662)
    Functional Studies - HDAC2 protein (Active) (ab101662)
    The specific activity of ab101662 was determined to be 4180 RLU/min/ng.

References

ab101662 has not yet been referenced specifically in any publications.

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