Recombinant Human HDAC2 protein (ab101662)
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- References
- Protocols
Description
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Product name
Recombinant Human HDAC2 protein
See all HDAC2 proteins and peptides -
Purity
> 70 % SDS-PAGE.
The purity of ab101662 was determined to be >70% by densitometry. -
Expression system
Baculovirus -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
92 kDa including tags -
Amino acids
1 to 488
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab101662 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride
General Info
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Alternative names
- D10Wsu179e
- HD 2
- HD2
see all -
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. -
Tissue specificity
Widely expressed; lower levels in brain and lung. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily. -
Post-translational
modificationsS-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching. -
Cellular localization
Nucleus. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human HDAC2 protein (ab101662)The purity of ab101662 was determined to be >70% as determined by densitometry.
Approximate molecular weight is ~92 kDa (with tag). -
Functional Studies - Recombinant Human HDAC2 protein (ab101662)The specific activity of ab101662 was determined to be 4180 RLU/min/ng.
Datasheets and documents
References
ab101662 has not yet been referenced specifically in any publications.
