Recombinant human HDAC3 protein (ab101663)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human HDAC3 protein
See all HDAC3 proteins and peptides -
Biological activity
Specific activity 125 RLU/min/ng.
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Purity
> 70 % SDS-PAGE.
ab101663 is >70% pure as assessed by densitometry. Affinity purified. -
Expression system
Baculovirus infected insect cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
83 kDa including tags -
Amino acids
1 to 428
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab101663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- HD3
- HDAC 3
- HDAC3
see all -
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily. -
Post-translational
modificationsSumoylated in vitro. -
Cellular localization
Nucleus. - Information by UniProt
Images
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The specific activity of HDAC3 (ab101663) was determined to be 144 RLU/min/mg as per activity assay protocol
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SDS PAGE analysis of ab101663
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SDS PAGE analysis of ab101663
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The specific activity of ab101663 was determined to be 125 RLU/min/ng.
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The purity of HDAC3 was determined to be >70% as assessed by densitometry.
The approximate molecular weight is ~83 kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab101663 has not yet been referenced specifically in any publications.