Description

  • Product name

    Recombinant human HDAC4 protein
  • Biological activity

    The Specific activity of ab104029 was determined to be 60 RLU/min/ng.
  • Purity

    > 95 % SDS-PAGE.
    Purity was determined to be >95% by densitometry.
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      77 kDa including tags
    • Amino acids

      612 to 1084

Specifications

Our Abpromise guarantee covers the use of ab104029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • AHO3
    • BDMR
    • EC 3.5.1.98
    • HA6116
    • HD 4
    • HD4
    • HDAC 4
    • HDAC A
    • HDAC4
    • HDAC4_HUMAN
    • HDACA
    • Histone deacetylase 4
    • Histone Deacetylase A
    • KIAA0288
    see all
  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
  • Tissue specificity

    Ubiquitous.
  • Involvement in disease

    Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 2 subfamily.
  • Domain

    The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    modifications

    Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
    Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
  • Cellular localization

    Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
  • Information by UniProt

Images

  • Kinase Assay demonstrating specific activity of ab104029.
  • SDS-PAGE showing ab104029 at approximately 77kDa.

References

ab104029 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Abcam's HDAC4 ab104029 is tested for activity using Promega's HDAC-Glo(TM) system. Here is a link: http://www.promega.com/resources/protocols/technical-manuals/101/hdac-glo-i-ii-assay-and-screening-system-protocol/ I hope this helps. If you have any other questions, please contact us.

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