Description

  • Product name

    Recombinant human HDAC6 protein (Active)
    See all HDAC6 proteins and peptides
  • Biological activity

    Specific Activity: ≥350 pmol/min/µg.
    Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 μM BPS HDAC substrate 3, and HDAC6 (3.1 – 200 ng). Incubation condition: 30 min at 37ºC followed by developing for 15 min at room temperature.
  • Purity

    > 65 % SDS-PAGE.
    Protein was purified by affinity chromatography and gel filtration.
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      159 kDa including tags
    • Tags

      GST tag N-Terminus
    • Additional sequence information

      BC069243.

Specifications

Our Abpromise guarantee covers the use of ab42632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Inhibition Assay

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.63% Tris HCl, 20% Glycerol, 0.64% Sodium chloride, 0.0165% Potassium chloride, 0.04% Tween, 0.0462% DTT

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CPBHM
    • FLJ16239
    • HD 6
    • HD6
    • HDAC 6
    • HDAC6
    • HDAC6_HUMAN
    • Histone deacetylase 6
    • Histone deacetylase 6 (HD6)
    • JM 21
    • JM21
    • KIAA0901
    • OTTHUMP00000032398
    • OTTHUMP00000197663
    • PPP1R90
    • Protein phosphatase 1 regulatory subunit 90
    see all
  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 2 subfamily.
    Contains 1 UBP-type zinc finger.
  • Post-translational
    modifications

    Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
    Sumoylated in vitro.
  • Cellular localization

    Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
  • Information by UniProt

Images

  • Specific Activity: ≥350 pmol/min/µg.
    Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 μM BPS HDAC substrate 3, and HDAC6 (3.1 – 200 ng). Incubation condition: 30 min at 37ºC followed by developing for 15 min at room temperature.
  • 4-20% SDS-PAGE - Recombinant human HDAC6 protein (Active) (ab42632).

    Lane 1: 2µg HDAC6.

    Lane 2: MW marker.

References

ab42632 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Recombinant human HDAC6 protein (ab42632) has a GST tag at N-terminal end.

Read More

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