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Dear Sir/Madam, we are looking at Human Heparan Sulfate Measurements in cultured fibroblasts. We usually use your anti-Heparan Sulfate Proteoglycan 2 antibody- A74 (ab23418) and we would like to realize a range with a recombinant HSPG2 protein in order to quantificate the HSPG2 presents in our cells.
For that, we need to know which epitope of the HSPG2 protein is recognized by our product? Do you know, which recombinant protein could we use? We didn’t find it in the website
I am looking forward to your reply as soon as possible. Please have a look. Thank you very much.
Asked on Mar 28 2012
Thank you very much for contacting us and for your patience. I apologise for the delay.
We have contacted the originator of the Anti-Heparan Sulfate Proteoglycan 2 antibody [A74]ab23418and enquired about epitope details. Unfortunately, there are no more details available than what is already stated on the datasheet: The epitope is located in domain V of Perlecan.
We unfortunately have only one Heparan Sulfate Proteoglycan 2 protein available in our catalog: ab114285 is a recombinant fragment of Human Heparan Sulfate Proteoglycan 2, amino acids 25-134.As thisrange of amino acids is different from domain V, ab114285 is therefore not a suitable option for your experimental setup.
We aim to provide as much information as possible to customers, so I am sorry that this has not been possible on this occasion.Please do not hesitate to contact us again with other needs or with any questions about our products.
Answered on Mar 28 2012