Recombinant Human Hepatitis C Virus (mutated D168V) protein (ab198068)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 53% SDS-PAGE
- Tags: His-DDDDK tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human Hepatitis C Virus (mutated D168V) protein -
Purity
>= 53 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MDYKDDDDKHHHHHHGCVVIVGRIVLSGSGSITAYAQQTRGLLGCIITSL TGRDKNQVEGEVQIVSTATQTFLATCINGVCWTVYHGAGTRTIASPKGPV IQMYTNVDQDLVGWPAPQGSRSLTPCTCGSSDLYLVTRHADVIPVRRRGD SRGSLLSPRPISYLKGSSGGPLLCPAGHAVGLFRAAVCTRGVAKAVVFIP VENLETTMRS -
Predicted molecular weight
22 kDa including tags -
Modifications
mutated N168V -
Tags
His-DDDDK tag N-Terminus -
Additional sequence information
Fusion protein corresponding to the serine protease NS3 (a.a. 3-181) and cofactor NS4A (a.a. 21-32) from Hepatitis C virus genotype 1a with Asp-to-Val mutation on a.a. 168
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab198068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.7% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- Genome polyprotein
- HCV
- Hepatitis C virus polyprotein
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Relevance
Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha. -
Cellular localization
Virus surface
Images
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SDS-PAGE - Recombinant Human Hepatitis C Virus (mutated D168V) protein (ab198068)
4-20% SDS-PAGE analysis of 1.6 µg ab198068 with Coomassie blue staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab198068 has not yet been referenced specifically in any publications.