Key features and details
- Expression system: Wheat germ
- Suitable for: SDS-PAGE, WB, ELISA
Product nameRecombinant Human Hes1 protein
See all Hes1 proteins and peptides
Expression systemWheat germ
Protein lengthProtein fragment
SequenceKSSKPIMEKRRRARINESLSQLKTLILDALKKDSSRHSKLEKADILEMTV KHLRNLQRAQMTAALSTDPSVLGKYRAGFSECMNEVTRFLSTCEGVNTEV RTRLLGH
Predicted molecular weight37 kDa including tags
Amino acids36 to 142
Our Abpromise guarantee covers the use of ab114192 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.79% Tris HCl, 0.3% Glutathione
FunctionTranscriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
DomainHas a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab114192 has not yet been referenced specifically in any publications.